| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,726,640 | 0 | T | A | 56.0% | 4.5 / 34.1 | 25 | N360K (AAT→AAA) | xylG | D‑xylose ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/10); new base A (14/0); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCT > NZ_CP009273/3726554‑3726717 | cAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATAtt < 1:538292/90‑1 (MQ=255) cAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTAcc < 2:120402/90‑1 (MQ=255) tCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAAAATTTCCCCccccg > 2:486008/1‑90 (MQ=255) aGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTTGGAAAAAAATTACCCCCGCCGCCCTcc > 2:671744/1‑89 (MQ=255) ggggATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTGAAAAAATTACCCCCCCCCCACTCaa > 1:38203/1‑90 (MQ=255) ggggATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGGAAAAAAATTACCCTCCCCGCCCTcca > 2:770880/1‑88 (MQ=255) ggggATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGGTGGGAAAAAAATTAACCCCCCCCCACCCaa > 1:19921/1‑90 (MQ=255) ggATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATa < 1:551105/90‑1 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAatat < 2:613501/67‑1 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAAAATTACCCTCCCCGCACTCAATAAAt > 1:433496/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGGAAAAAAATTTCCCCCCCCGCCCTCAAAAAAt > 2:572056/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGGAAAAAAATTAACCCCCCCGCACTCAATAAAt > 2:542842/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCCCGCCGCACTCAATAAATTTACCgg > 2:4927/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAAAATTACCCCCCCCGCACTCAATAAATTTAcccg > 2:382599/1‑88 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGGAAAAAAAATAACCCCCCCCCCCCCAAAAAATTTTCCgg > 1:671559/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGGTGGGAAAAAAATTACCCCCCCCGCCCCCAATAAAATTTCCgg > 2:665889/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGGTGGGAAAAAAATTAACCCCGCCCCACTCCATAAATTTaaccg > 1:58550/1‑86 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 2:433496/90‑1 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 1:382599/90‑1 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 2:584267/90‑1 (MQ=255) cAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCa < 2:243729/90‑1 (MQ=255) aGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGTGGGGAAAAAAATACCCCCCCCCCCCCCCAAAAAATTTCCCGG‑GGGGTTAGCCAg > 1:646555/1‑90 (MQ=255) cATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGc < 1:4927/90‑1 (MQ=255) gTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCaaaaa < 1:508808/90‑1 (MQ=255) aaTGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATg < 1:542841/90‑1 (MQ=255) ggTTGGGAAAAAAATTACCCCCCCCGCACCCAAAAAAATTAACGGGGGGAATAACCAGCTTTGGGAAGCGGCAGAGCAAAAAAATATTCt > 1:447171/1‑90 (MQ=255) | CAGCAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGC‑GGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTCT > NZ_CP009273/3726554‑3726717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |