| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 58.3% | 3.8 / 12.6 | 12 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTT > NZ_CP009273/535944‑536098 | aTCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTTTCCGGTTAAGGGGTGTTTTTGTTCCAttttt > 2:839667/1‑89 (MQ=255) tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTTAGGGGGGTTTTTTTTTAAtttttc > 1:108173/1‑88 (MQ=255) gTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTTAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGC‑GCAAGc > 1:124599/1‑90 (MQ=255) tAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGcc < 1:704818/90‑1 (MQ=255) tCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGt < 1:432105/90‑1 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGGGATTTTCCCGGTAAGGGGGGTTTTTGTTTAATTTTTCAGTCCGCCGC‑GGAAGCCTGttt > 1:829073/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGC‑GGGCATTTTCCGGTTTAGGGGGGGTTTTTTTTAATTTTTCGGTCCGCCGCGGAAAGCC‑GGTATCAggg > 1:677076/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCCATTTTTCCGTCAGCCCC‑GGAAACCCGTTTTCAggg > 1:97982/1‑89 (MQ=255) tGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGTTATCAGGAt < 1:743681/90‑1 (MQ=255) aTGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGTTATCAGGATTTCa < 2:278480/90‑1 (MQ=255) cGTCCTTGCGGC‑GGGCATTCTCCGGTTTAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGC‑GAAAGCCTGTTTTTAGGGTTTTTGTggg > 1:102619/1‑89 (MQ=255) tAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGtt > 2:322661/1‑90 (MQ=255) | ATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGC‑GGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGC‑GAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTT > NZ_CP009273/535944‑536098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |