Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,631,337	G→A	W112* (TGG→TGA)	pitA →	inorganic phosphate transporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,631,337	0	G	A	100.0%	239.9 / NA	62	W112* (TGG→TGA)	pitA	inorganic phosphate transporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/21);  total (41/21)

TGCTGCTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGGTACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGA  >  NZ_CP009273/3631252‑3631416                                                                                      |                                                                                tgctgcTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACt                                                                             <  2:26444/90‑1 (MQ=255)  gctgctTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCAGGAACCTGGGTACCTGATACtt                                                                            <  2:67530/90‑1 (MQ=255)       ttAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGtt                                                                       >  2:307173/1‑90 (MQ=255)        tAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGttt                                                                      <  2:784855/90‑1 (MQ=255)        tAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGttt                                                                      <  2:156954/90‑1 (MQ=255)               ggATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCa                                                               >  1:402116/1‑90 (MQ=255)               ggATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCa                                                               >  2:624039/1‑90 (MQ=255)               ggATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCa                                                               >  2:322523/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:114090/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:395440/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  2:361719/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:351262/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:825365/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:224890/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAg                                                          >  1:201923/1‑90 (MQ=255)                    gTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGGTTACCTGCATCCAg                                                          >  1:562593/1‑90 (MQ=255)                     tctcATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         <  2:395440/90‑1 (MQ=255)                     tctcATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         <  1:530711/90‑1 (MQ=255)                     tctcATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         <  2:825366/90‑1 (MQ=255)                              cTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         <  2:335607/81‑1 (MQ=255)                              cTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         >  1:335607/1‑81 (MQ=255)                              cTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACg                                                >  1:625716/1‑90 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGTTTTACCTGCATCCAGCTCTCATACGc                                               >  2:176165/1‑90 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGttt                                                                      <  1:300131/67‑1 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGttt                                                                      >  2:300131/1‑67 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGc                                               >  2:441782/1‑90 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGc                                               >  1:65848/1‑90 (MQ=255)                               ttGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGc                                               >  2:48481/1‑90 (MQ=255)                                tGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCt                                              >  1:124115/1‑90 (MQ=255)                                tGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCt                                              >  2:330443/1‑90 (MQ=255)                                tGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCt                                              >  2:645033/1‑90 (MQ=255)                                tGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCt                                              >  2:202590/1‑90 (MQ=255)                                         gtTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGgcgc                                     <  2:432882/90‑1 (MQ=255)                                         gtTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGgcgc                                     >  1:161043/1‑90 (MQ=255)                                          ttCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGcgcg                                    >  1:830131/1‑90 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATCCTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGa                                   <  2:562594/90‑1 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACtt                                                                            <  1:141848/49‑1 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACtt                                                                            >  2:141848/1‑49 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGa                                   <  1:266285/90‑1 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGa                                   >  2:266285/1‑90 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGa                                   >  2:172952/1‑90 (MQ=255)                                           tctcTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGa                                   >  1:512367/1‑90 (MQ=255)                                            ctctATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         >  1:356392/1‑67 (MQ=255)                                            ctctATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGc                                                         <  2:356392/67‑1 (MQ=255)                                                 tGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCg                             >  2:812718/1‑90 (MQ=255)                                                   ttGCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCggg                           >  2:199146/1‑90 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         <  2:403807/90‑1 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         <  2:679360/90‑1 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         >  1:156622/1‑90 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         >  2:335625/1‑90 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         <  1:335625/90‑1 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         <  2:156622/90‑1 (MQ=255)                                                     gCTGGCGGCGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         >  2:593462/1‑90 (MQ=255)                                                        ggcggcGATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTgg                      >  2:226090/1‑90 (MQ=255)                                                          cggcgATTATCTGGAACCTGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGtt                    >  2:432261/1‑90 (MQ=255)                                                                            tGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGgcgc                                     >  1:184704/1‑55 (MQ=255)                                                                            tGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGgcgc                                     <  2:184704/55‑1 (MQ=255)                                                                            tGGGTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTtgatga  >  2:381708/1‑90 (MQ=255)                                                                             gggTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTtgat    <  2:612511/87‑1 (MQ=255)                                                                             gggTACCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTtgat    >  1:612510/1‑87 (MQ=255)                                                                                 aCCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         <  2:694610/62‑1 (MQ=255)                                                                                 aCCTGATACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGAt                         >  1:694609/1‑62 (MQ=255)                                                                                      |                                                                                TGCTGCTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTTCTCTATGTTGCTGGCGGCGATTATCTGGAACCTGGGTACCTGGTACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGA  >  NZ_CP009273/3631252‑3631416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑