| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,028 | 0 | A | C | 70.6% | 5.2 / 12.1 | 17 | V556G (GTG→GGG) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/12); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496952‑497113 | gATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc > 1:699412/1‑90 (MQ=255) ccGCTGGCGCTTCTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTgg < 2:479703/89‑1 (MQ=255) gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCcagc < 2:316926/90‑1 (MQ=255) gTCTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCAACCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcagc < 2:538956/90‑1 (MQ=255) tCTTTTCTTCCCTACATTTTTTCCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCcagca < 1:212856/90‑1 (MQ=255) tCTTATCATCCCTACATTTTTTTAATTTTTTACACCCGGCACCCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCcagca < 2:527195/90‑1 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:424663/1‑90 (MQ=255) gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa > 2:578841/1‑90 (MQ=255) cccTACTTTTTTTTCATTTTTTCCACCCGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa < 1:70254/89‑1 (MQ=255) ccTACATTTTTTTCATTTTTTCCACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 1:522417/90‑1 (MQ=255) ccTACATTTTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTCTCCAGCAGTTCCAGCAt < 2:293225/90‑1 (MQ=255) tttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCg > 2:656345/1‑90 (MQ=255) tatTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTc < 2:229453/90‑1 (MQ=255) tACACCCGGCACCCCCCTTTTCCCCCCCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 2:606598/90‑1 (MQ=255) acacCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTc < 2:699412/90‑1 (MQ=255) cccttttCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc < 2:367908/86‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc > 2:366279/1‑90 (MQ=255) | GATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACC > NZ_CP009273/496952‑497113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |