| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 949,854 | 0 | A | C | 57.9% | 9.4 / 14.4 | 19 | G23G (GGT→GGG) | focA | formate transporter FocA |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/11); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGACGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAA > NZ_CP009273/949772‑949942 | agaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCct > 1:395962/1‑90 (MQ=255)agaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCct > 2:511050/1‑90 (MQ=255)agaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCct > 2:337375/1‑90 (MQ=255)agaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGGCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCct > 1:549039/1‑90 (MQ=255) tGCGTTTGAGATGAAACCCCGGGCGTTAACCCCCAGAAAAAAATTTTAAAGGGGTTTTTTGTTTTTTTTATAGCCCCCCGCCTCTTCGGc < 1:511050/90‑1 (MQ=255) tGCGTTTGAGATAAAACCCCCGGGGGAAACCCCAAGAAAAAAATTCTAAAGGGGTTTTTTCTTTTTTTTAAAGACCCCCGCCTCTTCGGc < 1:610283/90‑1 (MQ=255) tGCGATTGAGATAAAACCCCCGGGGGTAATCGCCAGAAAAAAAGTCTTAAGGGGTTGTTTCGTTGTTTTATAGACCCCCGCCTCTTCGGc < 1:337375/90‑1 (MQ=255) tGCGATGGAGATAAAACCCCGGGGGGTAACCGCCAGAAAAAATTTCTAAGGGGGTTTTTTCGTTTTTTAAAAGACCCCCGCCTCTTCGGc < 1:616996/90‑1 (MQ=255) gAAAACCCCGGCGGTAATCGCCAGATAAAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGCCACCCGCCTCTTCGGCCACTTTGGCCAt < 2:283372/90‑1 (MQ=255) gAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCAt > 2:670304/1‑90 (MQ=255) cgggggTAATCGCCAGATAAAAAGTCTTAGGGGGTTTTTTCGTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGcagcag < 1:545612/90‑1 (MQ=255) cggcggTAACCGCCAGATAGAAAGTCTTAAGCGGTTGTTTCGTTGTTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGcagcag < 2:219601/90‑1 (MQ=255) tCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa > 1:219601/1‑90 (MQ=255) ttAAGGGGTTTTTTCGTTGTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCa < 2:764924/90‑1 (MQ=255) ttAAGCGGTTTTTTCTTTGTTTTAAAAACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCa < 1:670304/90‑1 (MQ=255) ttttttCGTTCTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTcac < 2:549039/87‑1 (MQ=255) tttGTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAct < 2:36016/89‑1 (MQ=255) gTTTCTTTATACACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAAct < 2:73363/90‑1 (MQ=255) tttATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctctc < 2:240594/90‑1 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa > 2:177891/1‑90 (MQ=255) | AGACGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAA > NZ_CP009273/949772‑949942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |