| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,991 | 0 | A | C | 53.8% | 8.9 / 12.7 | 14 | G76G (GGT→GGG) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTT > NZ_CP009273/1377905‑1378074 | ttGTGCGACGTTTGTAATATCACGTCGAACCCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTcccc < 2:312009/90‑1 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgc > 1:405001/1‑90 (MQ=255) aCGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAg > 1:240577/1‑90 (MQ=255) ttttAATATCCCGTAGAAACTTTTTTCTTCAGTTTGGGGGGTGTTGGCAGAAAAACCTTCAGAAAAAAACGGATTCCCCGCCGTAGtttt < 1:543101/87‑1 (MQ=255) tttaaaTATCACGACGAAACTTTTTTTTTCCTTTTGGGGGGTGTGGGAATAAAAAACTCCAGAAAAAAAGGGATTCCCCGCCGTAGtttt < 1:409426/86‑1 (MQ=255) tatCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGa > 2:781516/1‑90 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 2:566739/1‑90 (MQ=255) cccGTCAAACCTTTCTTCTTCAGTTTTGGGGGTGATGGAATGAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACg < 2:415595/88‑1 (MQ=255) aacccTTCTTTTTCTGCTTGGGGGGTGTGGGCACCCAAATCTTCAAAAAAAAGCGCATTGCCCGCCGTAGTTTTGGTCGAACGGGTAGaa < 2:715755/87‑1 (MQ=255) aacccTTCTTCTTCGTCTTTGGCGGTTATGGCATGCAAACCTTAAGAAAAAAACGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGaa < 2:490801/87‑1 (MQ=255) cctttcttctccATTTTGGGGGGTGGGGGAATGAAAAATTTAAAAAAAAACCGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt < 1:353677/87‑1 (MQ=255) ccTTCTTCTTCAGCTTGGGGGGTGGTGGCATGAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 1:781516/90‑1 (MQ=255) tcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg > 1:335785/1‑90 (MQ=255) tcAGCTTGGGGGGTGATGGCATGCAAAACTTCAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATg < 2:335785/90‑1 (MQ=255) cGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGtt < 2:240577/90‑1 (MQ=255) | TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTT > NZ_CP009273/1377905‑1378074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |