| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,548,257 | 0 | T | G | 56.2% | 6.5 / 11.1 | 16 | Y557S (TAC→TCC) | maeA | malate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGC > NZ_CP009273/1548174‑1548339 | gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTgg > 2:61770/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCgg > 2:387767/1‑90 (MQ=255)gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGGCGCGGGATTCgg > 2:206156/1‑90 (MQ=255) gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCtttc > 1:728623/1‑88 (MQ=255) aaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCa < 2:690579/90‑1 (MQ=255) gagaGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCCTGCCCGaaaat > 2:428261/1‑88 (MQ=255) aaaTAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGCAGTGGGGGTATTCGGCTTGCGAGGAAATATTGTCa > 2:28712/1‑90 (MQ=255) ccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt < 1:29884/90‑1 (MQ=255) gggCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGGATTCTGCCTTCCCGAAAATTTCTTCAATAGCCCTTTGGAGGGCTTCGGCag > 2:358897/1‑90 (MQ=255) gcgcAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCagag > 2:599074/1‑90 (MQ=255) ggCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGGTTCGGCAGAGGgttt > 2:696904/1‑90 (MQ=255) gtttAGATGGGGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTAAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTc < 1:358897/88‑1 (MQ=255) gAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACg > 2:731371/1‑90 (MQ=255) gAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGGCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGccccc > 2:690177/1‑87 (MQ=255) gAGGTACGGCGGTAGGCGCGGGATTCGGCTTGCCAGAAAATATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCCCg > 1:221327/1‑90 (MQ=255) gTCGGGGTATTGGGCTTGCCAGAATTTCTCGTCACTGGCCTGTTGCAGGGTTTCGGCAGAGTTTTTCACCGCCACGCCTTGCTGCTgcgc < 1:648905/90‑1 (MQ=255) | GATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGC > NZ_CP009273/1548174‑1548339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |