| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 33,456 | 0 | C | A | 57.1% | 4.5 / 15.9 | 14 | T880T (ACC→ACA) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base A (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGC > NZ_CP009273/33383‑33541 | ccACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCccgc < 2:49272/90‑1 (MQ=255) gtcccGTTGGAAAAGGTGGCGGCGGGTGTGATGCCGGGCAACCCGTTGGTTGAGCAGGGCGTAACAAAAGAATTTACCCCGCCGtactac < 1:76130/87‑1 (MQ=255) gtcccGCGGGAAAAAGTGGCGGCGCGCGTGATGGCGGGAAAATCGCTGGCTGAGCGGGGCGTAACAAAAGAAGTTATCCCGCCGtactac < 1:167998/87‑1 (MQ=255) gTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGGAACCAAAGAAGTTATCCCGCCGtactac > 2:688940/1‑90 (MQ=255) ccGCTGGCAAAGGGGGCGGCGCGCGGGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACAAAGGAAGTTATCCCGCCGTACTACTCg < 2:374887/90‑1 (MQ=255) ccGCGGGAAAAGGGGGCGGCGCGCGTGATGGCGGGAAAACCGCTGGCTGAGCAGGGCGAAACAAAAGAAGTTATCCCGCCGTACTACTCg < 1:685816/90‑1 (MQ=255) tGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGa > 1:205574/1‑90 (MQ=255) aaaGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAaagaa < 2:205574/90‑1 (MQ=255) cgcgagTGATGGCCGAAAACCCGGTGGCTGAGAGGGGGGTAACAAAAAAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGtgctgc < 1:288772/90‑1 (MQ=255) gTGAGGGCGGGAAAACCGCGGGCGGAGCGGGGCGAAACAAAAAAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTc < 1:179555/90‑1 (MQ=255) ggcAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTc > 1:270882/1‑90 (MQ=255) aaaTCGCGGGCGGGGCGGGGCGAACCAAAAGAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCg < 1:173426/90‑1 (MQ=255) ggAGCGGGGCGAAACAAAAAAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGAccc < 1:593502/89‑1 (MQ=255) tAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGc > 2:568057/1‑90 (MQ=255) | CCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGC > NZ_CP009273/33383‑33541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |