| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,152 | 0 | C | A | 56.2% | 10.1 / 23.7 | 17 | A321S (GCC→TCC) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base A (0/9); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC > NZ_CP009273/2356069‑2356234 | cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATg > 2:806848/1‑90 (MQ=255) ggcgCGATAGTGCCGTCTGGCGTGGCTAGCGCAGAGGTGAGATGCTCGTTCTCGAAACCAG‑AAGCAGGCAGCGCCAGCCCGGGAAAATgc > 1:268405/4‑89 (MQ=255) agCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGtt < 2:731951/90‑1 (MQ=255) aggTGCCGTCGGGGGGGGCTAGCGAAAAGTTGAGATGCTCGTTCCCAAACCCCA‑AAACAGCCAGTGCCAGCCCGGAAAAATGGTTGGccc < 1:119499/88‑1 (MQ=255) gCTGGCGAAAGGTGGAGATCCTCTTTCCCAAACCCGG‑AACCAGCCGGGGCCGGCCGGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAg < 1:764975/90‑1 (MQ=255) gcgcAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg > 1:587963/1‑90 (MQ=255) cgcAAAGTGGAGATCTTCTTTCTCAAACCCCA‑AAACAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcgc < 2:485169/90‑1 (MQ=255) gagaTGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgcgc > 2:710371/1‑90 (MQ=255) agaaCCCGAAAACAGCCGGTCCCAGCCGGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTcctcct < 2:487901/88‑1 (MQ=255) ccG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg < 2:195666/90‑1 (MQ=255) g‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGa > 2:463251/1‑90 (MQ=255) acccAGCGCCAGCCCGGAAAAATGGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCAc < 1:794693/88‑1 (MQ=255) gCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACt > 2:153115/1‑90 (MQ=255) ccgccccGGAAAATTGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACg < 1:705568/86‑1 (MQ=255) ccAGCCGGAAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACg < 1:810838/90‑1 (MQ=255) aGCCGGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGcc < 2:184071/90‑1 (MQ=255) aGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGcc < 2:587963/90‑1 (MQ=255) | CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC > NZ_CP009273/2356069‑2356234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |