| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,679,388 | 0 | T | C | 55.6% | 11.3 / 21.8 | 18 | E1049G (GAG→GGG) | bcsC | cellulose synthase complex outer membrane protein BcsC |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base C (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACC > NZ_CP009273/3679302‑3679467 | gCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc > 2:323696/1‑90 (MQ=255)gCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc > 2:328835/1‑90 (MQ=255) ggtcggGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCa < 1:323696/90‑1 (MQ=255) catcaGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTc > 2:555177/1‑90 (MQ=255) ccggCGGATACCGCGGCATGTTTTGGGGGCGTGAATGCGCCCACGACCCAGACGCCCCCAGCCCCCACGACCATTTTTCCGTGCGCTccc < 1:297569/87‑1 (MQ=255) gCGGATACCGCGGCTTGTTTTTGGGGCGTGAATGCGCCCCCGAGCCAGCCGCCCCCAGCCCCCACGACCAGTTTTCCGTGCGCTCCCGcc < 1:115898/90‑1 (MQ=255) ggATACCGCGGCTTGTTTTTGGGGCGTAAAGGCCCCCCCGAGCCAGCCCCCCCCACCCCCCACGACCAGTTTCCCGTCCCCCCCCGCcac < 1:135931/90‑1 (MQ=255) cgGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCgg > 1:294089/1‑90 (MQ=255) cATGGTTTTGGTGCGTGAATGCGCCCCCGAGCCAGACGCACCCAGCCCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTAt < 2:294089/90‑1 (MQ=255) ggggCGTGAGGGCGCCCCCGACCCAGACCCCCCCGCCCCCCCCGCCCATTTTCCCGGGCCCCCCCGCCACATCACCGGTATGGCAAACGa < 1:729966/87‑1 (MQ=255) tGCGTGAAGGCGCCCACGACCCAGCCGCCCCCAGCCCCCACGACCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACa < 2:344153/90‑1 (MQ=255) gCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCt > 1:743327/1‑90 (MQ=255) gACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGc > 1:771976/1‑90 (MQ=255) ccACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCgg > 2:271508/1‑90 (MQ=255) aCGAGCCAGCCGCACCCAGCCCCCACGCCCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc < 2:13231/90‑1 (MQ=255) ccGCCCCCACCCCCCACGCCCTGTTTCCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAc < 2:110589/89‑1 (MQ=255) ccGCCCCCACCCCCCACGCCCATTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAc < 2:59414/89‑1 (MQ=255) cGCCCCCAGCCCCCACGACCATTTTTCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc < 2:276821/90‑1 (MQ=255) | GCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACC > NZ_CP009273/3679302‑3679467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |