Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,991,5290AT58.3% 3.5 / 12.7 12T63S (ACC→TCC) uvrDDNA helicase II
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base T (7/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCA  >  NZ_CP009273/3991446‑3991607
                                                                                     |                                                                              
aGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGG‑C‑TTTTTTcaaca                                                                          >  1:404669/1‑90 (MQ=255)
    gcgcgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGAGG‑GTTTTCCaaaaaaa                                                                       >  2:680436/1‑89 (MQ=255)
    gcgcgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGG‑G‑GGTTTCCAACaaaac                                                                      >  1:5374/1‑88 (MQ=255)
    gcgcgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGG‑CGGTTTTCCAAAAAAg                                                                       >  1:660861/1‑90 (MQ=255)
       cgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAgcggc                                                                   <  1:542791/90‑1 (MQ=255)
           cTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAgcggcggcg                                                               <  1:454248/90‑1 (MQ=255)
                              ggTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GGTTTCCAACAAAAAGGCGGCGGGGAGGCGTCATCGTATCg                                            >  2:308554/1‑90 (MQ=255)
                                 tgatgaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGc                                         <  1:381962/90‑1 (MQ=255)
                                              aaaCTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCAc                            <  2:110280/90‑1 (MQ=255)
                                                                  ttATGGCGGTGA‑C‑GTTTTCCAACAAAACGGCGGCGGGGGTGGGTCATCGTATCGGGGAACCGATGGGGACGAGCCCGGGGGGGATGTggg        >  2:33308/1‑90 (MQ=255)
                                                                  ttATGGCGGTGA‑C‑GTTTTCCAACAAAACGGCGGCGGGGGAGCGGCATCGTATCGGGGAACCGAGGGGGACGAGCCCGGGCGGGGTGTggg        >  2:761957/1‑90 (MQ=255)
                                                                        cGGTGA‑C‑GTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCa  <  1:690394/90‑1 (MQ=255)
                                                                                     |                                                                              
AGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGA‑C‑GTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCA  >  NZ_CP009273/3991446‑3991607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑