| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,031 | 0 | T | G | 52.9% | 11.1 / 14.6 | 17 | V211G (GTT→GGT) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (5/3); new base G (9/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTA‑C‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTC > NZ_CP009273/699944‑700120 | gTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTA‑C‑GGgtt > 2:210302/1‑90 (MQ=255) ctgATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTA‑C‑GGTTTTCCAc < 1:494563/90‑1 (MQ=255) gATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTTCACgg > 2:494563/1‑90 (MQ=255) cTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTA‑C‑GGtt < 2:864185/69‑1 (MQ=255) aGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGTTTTTCACGGTGACATTAACCGCTTCTATg > 1:358857/1‑90 (MQ=255) aGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCACGGGGACATTTACCGCTTCTATg > 1:835919/1‑90 (MQ=255) aGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCACGGGGACATTAACCGCTTCTATg > 2:311155/1‑90 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCGCCAACGCGGCGGGGACC‑GGTTTTCC‑CGGGGAAATTTACCGCTTCTATTCCggg > 1:876854/1‑89 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑CGGGTTTTCCCCGG‑GACATTAACCCCTTCTATGCCggg > 1:666597/1‑89 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCACCGGGACATTAACCGCTTCTATGCCggg > 2:691078/1‑89 (MQ=255) acacCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCACCGGGACATTAACCGCCTCTATGCCGGGGACg > 1:887726/1‑90 (MQ=255) tCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCACGGTGACATTAACCGCTTCTATGCCGGGGACGGCACcg > 2:854368/1‑90 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑G‑GGTTTTCCCCGGGGACATTAACCGCCTCTTTTTCGGTGGCGGCTCcacg > 2:925546/1‑90 (MQ=21) ggTTCCAGATTGGTGAATTCACCCACGCGGCGGGTA‑C‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGa < 1:250569/90‑1 (MQ=255) gTTCCAGATTGGTGAATTCACCAACGCGGCGGGGA‑C‑GGGTTTCCCCGGGGACATTAACCGCTTCTATGCCGGGGACGGCACCCCgggggt > 2:581216/1‑88 (MQ=255) ggTGAATTCACCAACGCGGCGGGTA‑C‑GGGTTTCCACGGGGACATTAACCGCTTCTATGCCGGGGACGGCACCCCGGGGGTGTTCATGTcc > 2:792101/1‑90 (MQ=255) aTTCACCAACGCGGCGGGTA‑C‑GGTTTTCCACGGTGACATTAACCGCTTCTATTCCGGAGACGGCACCGCGGGGAAGTTCCTGTTCAGctt > 2:818481/1‑90 (MQ=255) tCACCAACGCGGCGGGGA‑C‑GGGTTTCCACCGGGACATTAAACGCTTCTATGCCGGGGGCGGGACCGCGGGGGTGTTTATGTCCGGcttct > 1:411434/1‑90 (MQ=255) cAACGCGGCGGGTA‑C‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTccc < 1:581216/90‑1 (MQ=255) ttttCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTc < 2:193524/90‑1 (MQ=255) | GTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTA‑C‑GGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTTCGGTC > NZ_CP009273/699944‑700120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |