| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 892,805 | 0 | T | G | 54.5% | 11.1 / 12.9 | 11 | V89G (GTG→GGG) | potI | putrescine ABC transporter permease PotI |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACG > NZ_CP009273/892732‑892890 | gttggttTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGCTGGGGCGCTTTGGc > 2:500614/1‑90 (MQ=255) ggttTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGCTGGGGCGGTTTTGGAgg > 2:849772/1‑90 (MQ=255) tGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTGGCAGGGTTCGCGGAt > 2:351848/1‑90 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGCTGGGGGGCTTTGGCAGGGTTTGCGGATc > 2:520139/1‑90 (MQ=255) gtgCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCt < 1:849772/90‑1 (MQ=255) gtgCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGGGCTGGGGCGCTTTGGCAGGGTTCGCGGATCAAAA‑GGCTTTGCCt > 2:191317/1‑90 (MQ=255) cgATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGcgccgc < 1:140996/90‑1 (MQ=255) cTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTa < 1:703838/90‑1 (MQ=255) tGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGt < 2:630066/90‑1 (MQ=255) ggcggTGGGGCTGGTGGGCTTTGGGAGGGTTCGCGGATCAAAT‑GGGTTTTCCTTTTTTATCACCCCGCCGCTGGGAATGCCAGATGtcat > 1:394955/1‑90 (MQ=255) gcggTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGtcatc < 2:394955/90‑1 (MQ=255) ggtgggGCTGGTGCGCTTTGGCAGGGTTCGCGGATCAAATGGGTTTTGCCTTT‑TGATCACCCCCCCGCCGGGAATGCCAGACGTCATCAc > 1:630066/1‑90 (MQ=255) gtggtgCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACg < 1:520139/90‑1 (MQ=255) | GTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT‑GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACG > NZ_CP009273/892732‑892890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |