Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC53.3% 23.0 / 41.2 30V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (11/3);  new base C (0/16);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 6.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-05
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537
                                                                                         |                                                                                         
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  1:763798/1‑90 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  1:841835/1‑90 (MQ=255)
  ccGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCAcc                                                                                         >  1:946673/1‑90 (MQ=255)
     cacaCCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCttt                                                                                      <  1:83566/90‑1 (MQ=255)
         ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAACCCCCTTTgcgc                                                                                  <  1:950432/90‑1 (MQ=255)
            gAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCtt                                                                               >  1:391349/1‑90 (MQ=255)
                 aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc                                                                          >  2:334966/1‑90 (MQ=255)
                      ttGATTCGTTGCCAGTTCGCTGACGCCAGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCcgccgc                                                                     >  2:709622/1‑90 (MQ=255)
                             gTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCtttt                                                              >  2:915472/1‑90 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTtgct                                                           <  2:437227/90‑1 (MQ=255)
                                            ccGCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCg                                               <  1:849492/89‑1 (MQ=255)
                                             cGCCCGTTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGt                                              <  1:176517/90‑1 (MQ=255)
                                              gCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                             <  1:19637/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTgcgc                                                                                  <  2:606526/48‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:462442/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgcgc                                                                                  >  1:606526/1‑48 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:763798/90‑1 (MQ=255)
                                                    ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                       <  2:32621/90‑1 (MQ=255)
                                                    ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                       <  1:231913/90‑1 (MQ=255)
                                                           aGCTTGCGCCCCTGTGGTGACAAATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                                <  1:772759/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  2:391349/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  2:494773/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  2:616496/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGAAAAATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:846069/90‑1 (MQ=255)
                                                               tGCGTCCCTGTGGTGAAAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACca                            <  2:872037/90‑1 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                       <  1:598661/78‑1 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                       >  2:598661/1‑78 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                           >  2:251402/1‑90 (MQ=255)
                                                                gCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACcat                           >  2:526649/1‑90 (MQ=255)
                                                                                aGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGt           >  1:740219/1‑90 (MQ=255)
                                                                                     aaTCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc      <  2:946195/90‑1 (MQ=255)
                                                                                     aaTCACCTTTTCGTCTTCACCCGAGGCGGCTTTGGCTGCATGAATGGCATCCCGCGAAGGTTACCCCATCATTAACATCCCCGGTccgcc      >  1:316005/1‑90 (MQ=255)
                                                                                         aCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgccg  >  2:567664/1‑90 (MQ=255)
                                                                                         |                                                                                         
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: