| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,840,799 | 0 | A | C | 53.3% | 15.5 / 18.4 | 15 | V601G (GTG→GGG) | hycC | formate hydrogenlyase subunit 3 |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGAGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCAGCCCGGCA > NZ_CP009273/2840710‑2840874 | gagaGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATca > 1:470282/1‑90 (MQ=255)gagaGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATca > 2:451137/1‑90 (MQ=255)gagaGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATca > 2:163063/1‑90 (MQ=255)gagaGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATca > 1:675293/1‑90 (MQ=255) gcagcGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATcaccac > 1:369877/1‑90 (MQ=255) gcagcGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATcaccac < 1:850645/90‑1 (MQ=255) gcggcgCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAg > 1:195731/1‑90 (MQ=255) gcggcgCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAg > 2:590698/1‑90 (MQ=255) cAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGc < 2:68801/90‑1 (MQ=255) tAACACCAGCGCCTGAATTAACGGATATAAACCCCTCATTCTCAGGCTCCTCGTGAAACAATAATCCCCACCAGTACCGCCAGTTCAAcc < 1:590698/90‑1 (MQ=255) tGAATTAAGGGATAAAAAACCCTCATTCTCAGGCTCCTCGTGAAACAATAATCCCCCCCAGTACCGCCAGTTCAACCAGCGCCATCcggc < 1:69230/90‑1 (MQ=255) tAACGGATATAAACCACTCATTCTCAGGCTCCTCGTAAAACAATAACCCCCCCCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAAc < 1:606314/90‑1 (MQ=255) aCGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCCCCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAg < 1:94333/90‑1 (MQ=255) ggATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCa < 2:798244/90‑1 (MQ=255) acTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTcccc > 2:596347/1‑90 (MQ=255) ttCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCa > 2:512009/1‑90 (MQ=255) aGGCTCCTCGTGAAACAATAATCCCCCCCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGcc < 2:927573/90‑1 (MQ=255) ggCTCCTCGGGAAACAATAATCCCCCCCAGTCCCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCa < 1:12908/90‑1 (MQ=255) cctcGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCAGccc > 1:99383/1‑90 (MQ=255) gTGAAACAATAATCCCCCCCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCAGCCCGGCa < 2:675293/90‑1 (MQ=255) gTGAAACAATAATCCCCCCCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCAGCCCGGCa < 2:470282/90‑1 (MQ=255) | GAGAGCAGCGGCGCAACGGCAAATAACACCAGCGCCTGAATTAACGGATATAAAACACTCATTCTCAGGCTCCTCGTGAAACAATAATCACCACCAGTACCGCCAGTTCAACCAGCGCCATCCGGCGGAACAGCAACGCACTCCCCTCGCACTGCCAGCCCGGCA > NZ_CP009273/2840710‑2840874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |