| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,028 | 0 | A | C | 57.1% | 10.8 / 16.7 | 14 | V556G (GTG→GGG) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCA > NZ_CP009273/496939‑497114 | gCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTca < 2:468020/90‑1 (MQ=255) ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacca > 2:263012/1‑90 (MQ=255) ccTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCAcc > 1:871086/1‑90 (MQ=255) tttttATCATGCCTATATTTTTTTAATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc < 1:183942/87‑1 (MQ=255) ttCTTTTCTTCCCTACATTTTTTTCATTTTTTCCCCCCGGCACCCCCCGTTCCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCcagc < 1:587922/89‑1 (MQ=255) gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc > 2:605900/1‑90 (MQ=255) tGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGc > 2:490275/1‑90 (MQ=255) ccTACTTTTTTTTCATATTTTACATCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt < 2:701594/90‑1 (MQ=255) aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGt > 1:712199/1‑90 (MQ=255) ttttCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc < 2:63215/90‑1 (MQ=255) ttttCATTTTTTACACCCGCCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGc < 2:750066/90‑1 (MQ=255) tCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt > 1:63215/1‑90 (MQ=255) tACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 2:712199/90‑1 (MQ=255) tACACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCt < 2:314493/90‑1 (MQ=255) ccccGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc < 1:194442/88‑1 (MQ=255) gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacca > 1:396788/1‑90 (MQ=255) | GCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCA > NZ_CP009273/496939‑497114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |