| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 6,994 | 0 | A | C | 58.3% | 13.6 / 24.9 | 24 | G322G (GGT→GGG) | yaaJ | sodium:alanine symporter family protein |
| Reads supporting (aligned to +/- strand): ref base A (9/1); new base C (0/14); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCC > NZ_CP009273/6910‑7074 | cTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGc > 2:334922/1‑90 (MQ=255) tCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGcc > 1:627848/1‑90 (MQ=255) aaGGAACCCATTCCCCCCCCCATCCCCTTCTGAAAAAGCTGAATCCCTCCCAGCGGCTTGTGGTTTGTGCCTTTCCCCGCCAGTAATATc < 1:59687/89‑1 (MQ=255) ccATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATgg > 1:38416/1‑90 (MQ=255) aTTAGCCCCCGCACCCCCTTCTGGATAAGCTAAATCCCTCCCAGCGGCATGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGgcg < 2:167754/90‑1 (MQ=255) aGCCCCCGCCTCCCCTTGTGAAAAACCTAAATCCCTCCCGGCGGCATGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCtt < 1:437962/90‑1 (MQ=255) cccGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCg > 1:173723/1‑90 (MQ=255) cGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTa > 2:112327/1‑90 (MQ=255) gCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTAc > 1:854644/1‑90 (MQ=255) ccccccTTGTGGAAAACCAAAATCCCTCCCGGGGGCATGGGGGTTGTGCCTTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAg < 1:850644/86‑1 (MQ=255) cccTTCTGAAAAAGCTAAATCCCTCCCGGCGGCTTGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATg < 1:313952/89‑1 (MQ=255) ttCTGAAAAAGCTAAATCCCTCCCGGCGGCATGTAGTTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGAcc < 2:367637/90‑1 (MQ=255) cccTCCCAGCGGCATGTAGGTTGTCCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAAt < 2:166450/89‑1 (MQ=255) ccggCGGCTTGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 2:854644/87‑1 (MQ=255) ccAGGGGCGTGTGGTTTGTGCCTTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 2:626242/90‑1 (MQ=255) ccAGCGGAATGTAGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 1:250989/90‑1 (MQ=255) ggCTTGTGGTTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc < 2:828219/90‑1 (MQ=255) ggCCTGTAGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc < 2:698772/90‑1 (MQ=255) ggCATGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc < 1:501728/90‑1 (MQ=255) ggCAGGTAGTTTGTCCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc < 2:318953/90‑1 (MQ=255) gCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCt > 1:828219/1‑90 (MQ=255) tgtgCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATccc > 1:534227/1‑90 (MQ=255) gtgCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAggg < 1:493169/55‑1 (MQ=255) gtgCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAggg > 2:493169/1‑55 (MQ=255) | CTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCC > NZ_CP009273/6910‑7074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |