| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 62.5% | 8.9 / 15.7 | 16 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (10/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 | cGATACGCTGGAAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTAAAGAGGACAGAGAAACGGC‑GGGTaa < 1:483051/90‑1 (MQ=255) tGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGAt < 2:278847/90‑1 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTAATGGATCTT‑GGGttt > 2:489734/1‑90 (MQ=255) gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGATTTC‑GGGTTTTACATCTCCttttt > 1:491496/1‑89 (MQ=255) aCGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTcc < 1:171650/90‑1 (MQ=255) gTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGa < 1:265212/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCt < 1:308769/90‑1 (MQ=255) actacACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGGTTTCC‑GGTTTTTAAAATTCCTTTTTCGCGTTTTGGACCTTcc > 1:670976/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGATCTT‑GGGTTTTATATCTCCTTTTCCGGGATTGTGGCCTTCCGGAATGCgg > 2:868001/1‑90 (MQ=255) gagGACAGAGAAACGGC‑GGGGAAATTACTTGATCTC‑GGGTTTTAAATCTCCTTTTTCCGCATTTTGGACTTCCCTAAATCGGGGGAACTg > 2:696628/1‑90 (MQ=255) gagGACAGAGAAACGGC‑GGGGAAAATACTGGATCTC‑CGGTTTTTTATCTCCCTTTTCCGCCTTGTGACCCTCCCCAAAGCGGGGCAACTg > 2:640315/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTACTTGGTCTC‑GGGTTTTAAATCTCCTTTTTCGGCATTGTGGCCTTCCGTAATGCGGGGCAACAgcg > 2:82543/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTACTGGATCTC‑GGGTTTTTAATCTCCTTTTTCCGCATTGTGGCCTTCCCTAATGCGGGGGAACTgcg > 2:294797/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTACTGGATCTC‑GGGTTTTACATCTCCTTTTTCGGCATTGTGACCTTTCCTAATGCGGGGGAACTgcg > 1:229038/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTAATGGGTTTCGGGTTTTTAAATTTCTTTTTCC‑GCCTTTTGGCCTTTCCTAATGCGGGGCGACGgcg > 1:867100/1‑90 (MQ=255) gaAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACCTCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgct < 1:648583/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:757933/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:696628/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:867100/90‑1 (MQ=255) | CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |