| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,134,407 | 0 | A | C | 55.0% | 17.8 / 27.9 | 20 | G229G (GGT→GGG) | asmA | outer membrane assembly protein AsmA |
| Reads supporting (aligned to +/- strand): ref base A (5/4); new base C (0/11); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCG > NZ_CP009273/2134319‑2134495 | tttGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCAc < 2:336974/90‑1 (MQ=255) gTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTg > 2:75899/1‑90 (MQ=255) ggCGTTTATGTGCCTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGt < 1:144830/90‑1 (MQ=255) tcccACTGGGCCTGAAACCCCCCCTGCCCCTGAATCCCTTTTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAgcc < 1:336954/88‑1 (MQ=255) tcccACTGGGCCGGGAACCCCCCCCCCCCCTAAATCCCTTTTTTTGGAAAATCGGCCCCCTCCAACTGCCAGTTAATTTTTTCAATAgcc < 1:401325/88‑1 (MQ=255) cTGGGCCGGAAACCCCCCCTCCCCCTGAATCCTTTTTTTTGGAAAATCGGCCCCCTGCACCTGCCAGTTAATTTGTTCAATAGCCGCCGt < 1:546641/90‑1 (MQ=255) aCTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAgcc < 2:752117/73‑1 (MQ=255) aCTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAgcc > 1:752117/1‑73 (MQ=255) tgcccctgAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGTGGATAATCa > 2:218605/1‑90 (MQ=255) tgAATCCCTTGTTTTGGTAAATCGGCCCCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCa < 2:324315/90‑1 (MQ=255) cctttGTTTTGGAAAATCGGCCCCCTGCAACTGCCATTTATTTTGTTAAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtac < 1:86321/87‑1 (MQ=255) ccTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGt > 1:592445/1‑37 (MQ=255) ccTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGt < 2:592445/37‑1 (MQ=255) ttttttGGAAAACCGCCCCCCTGCAACTGCCAGTTATTTTGTTAAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgta < 1:275520/88‑1 (MQ=255) ttttGGAAAATCGCCCCCCTGCAACTGCCAGTTAATTTTTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCtaccgtacc < 1:72709/90‑1 (MQ=255) ggTAAATCGGCCCCCTGAAACTGCCAGTTAATTTGTTAAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTa < 2:493641/90‑1 (MQ=255) ggTAAACCGGCCCCCTGCAACTGCCAGTTAATTTTTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTa < 2:667606/90‑1 (MQ=255) aaaaTCGCCCCCCTGCAACTGCAAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAg < 1:223847/89‑1 (MQ=255) ccGGCCCCCTCCAACTGCCAGTTATTTTTTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGat < 1:179148/89‑1 (MQ=255) cACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCg > 1:892192/1‑90 (MQ=255) | TTTGGTTAAATGAAAGGCGTTTATGTGACTCCTGCCACTGGGCCTGGAAACTCCCCTGCCCCTGAATACCTTGTTTTGGTAAATCGGCACCCTGCAACTGCCAGTTAATTTGTTCAATAGCCGCCGTTAAATCATGCGGATAATCAGAAGCATCTACCGTACCGTTAAGGGATATCG > NZ_CP009273/2134319‑2134495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |