| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,216,154 | 0 | T | G | 56.2% | 8.0 / 14.5 | 16 | V164G (GTG→GGG) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216070‑2216236 | gcgAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTTTAACAACTCCCGCGGCTCGCTGGGGCAAc > 2:90839/1‑90 (MQ=255) aaCCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTTTAACAACTCCGGCGGCTCGCTGGGGCAACGTg > 2:372700/1‑90 (MQ=255) caggTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCg < 2:862501/87‑1 (MQ=255) catcGTGTATAGGCGCATCGGTCATCGGCGGTATTTTTTAAAAATCCCGCGGGCCGCTGGGGGAACGGGGCCCGGCGGATACCGaaacag > 1:862501/1‑87 (MQ=255) tcGTGTATAGGCGCATCGGTCATCGGCGGAATTTGTAAAAACTCCGGCGGGCCGCTGGGGCAACGGGGCCCCGCGGATACCGAAATGTCg > 1:309196/1‑90 (MQ=255) gtATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCCGCGGATACCGAAATGTCGtttt > 2:634426/1‑88 (MQ=255) ggCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTcgcg < 1:634426/90‑1 (MQ=255) gcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGt > 2:430529/1‑90 (MQ=255) gcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCGGCGTATACCCGAAAGTCGTTTTTTGCGGGt > 2:524843/1‑90 (MQ=255) cATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGAGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGta > 2:311560/1‑90 (MQ=255) aTCGGTCATCGGCGGTATTTTTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCCGCGTATACCGAAATGTCGTTATTTGCGCGgat > 2:750268/1‑90 (MQ=255) aTCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCCGCGGATACCGAAATGTCGTTATTTGCGCGtat > 2:750269/1‑90 (MQ=255) aTCGGTCATCGGCGGGATTTTTAAAAACTCCCGCGGCTCGCTGGTGCAAACGGGCCCCGCGGATACCGAAAAGTCGTTATTCGCGCGtat > 1:377134/1‑90 (MQ=255) caacTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCt > 1:536809/1‑90 (MQ=255) gcTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATc < 1:90839/90‑1 (MQ=255) tCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTg < 2:848257/90‑1 (MQ=255) | GCGAACCGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216070‑2216236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |