| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,594,920 | 0 | A | C | 61.1% | 9.6 / 12.3 | 18 | V176G (GTG→GGG) | ftsX | permease‑like cell division protein FtsX |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (0/11); total (4/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCA > NZ_CP009273/3594831‑3595001 | tccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca < 1:439944/90‑1 (MQ=255) tGCGTGATCCGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGttt < 2:139872/90‑1 (MQ=255) gATACGATCCCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCCCCACCGCCACTGCCGGAAGCGGGTTttcttc < 2:9885/90‑1 (MQ=255) gATACGACCCCGCAGCGTATTCAGTGATCCCGCCCCCGGAAAATCGAGTTCCGGGACCCCCACCGCCACTGCCGGAAGCGGGTTttcttc < 1:862660/90‑1 (MQ=255) cGCAGCTTATTCAGTGATTCCGCCCCCTGAAAATCGATTTTCGGGATCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATcc < 2:87218/90‑1 (MQ=255) gCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAgcg > 1:666550/1‑90 (MQ=255) gTATTCAGTGATTCCGCCCCCTGGAAATCGATTTTCGGGATCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca < 2:224971/90‑1 (MQ=255) gTATTCAGGGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca > 2:620708/1‑90 (MQ=255) tATTCAGTGTTTCCGCCCCCTGGAAATCGAGTTTCGGGATCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac < 2:381206/90‑1 (MQ=255) tATCCAGTGTTCCCGCCCCCGGAAAATCGTGTTTCGGCCCCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac < 1:473644/90‑1 (MQ=255) tGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac > 1:33961/1‑83 (MQ=255) tGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac < 2:33961/83‑1 (MQ=255) aTTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAac > 1:224971/1‑90 (MQ=255) ccccTGAAAATCGATTTTCGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacca < 1:282763/90‑1 (MQ=255) ccccTGAAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacca < 2:209943/90‑1 (MQ=255) tCGATTTTGGGGATCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAAc < 2:562838/90‑1 (MQ=255) gTTTCGGGATCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAc < 2:248151/90‑1 (MQ=255) ttggggACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAccc < 1:510247/87‑1 (MQ=255) tggggATCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCa < 1:160413/88‑1 (MQ=255) | TCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCA > NZ_CP009273/3594831‑3595001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |