Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,248,9020TG57.1% 7.9 / 13.8 14G393G (GGT→GGG) dinFMATE family efflux transporter DinF
Reads supporting (aligned to +/- strand):  ref base T (1/5);  new base G (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCT‑GGCATTAACCGTCTTTCTGGCG  >  NZ_CP009273/4248825‑4248977
                                                                             |                                                                            
tATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCGGGGTTTTTGCTGGCg                                                                  >  2:742969/1‑90 (MQ=255)
    tgctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGGGGCGCCGCCGGGTTTTTGCTGACGCTcc                                                              >  1:331879/1‑90 (MQ=255)
     gctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTTCGCTGACGCTCCt                                                             >  1:822985/1‑90 (MQ=255)
          aCGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGc                                                        <  1:196074/90‑1 (MQ=255)
             gCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTTCGCTGACGCTCCCTACGCTGc                                                     >  1:655522/1‑90 (MQ=255)
                      taGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTgg                                            <  1:536379/90‑1 (MQ=255)
                      taGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTgg                                            <  2:4828/90‑1 (MQ=255)
                        ggCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGt                                          <  2:372799/90‑1 (MQ=255)
                          cgcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTaa                                        <  2:581113/90‑1 (MQ=255)
                           gcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCGGGGTTTTCGCCGGCGCCCCCTACGCTGCCGGGGCGGGGGAAt                                       >  1:730593/1‑90 (MQ=255)
                           gcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGGGGCGCCCCCGGGTTTTCGCTGACGCTCCTTTCGCCGCCGGGGCTgggggaa                                       >  1:701091/1‑86 (MQ=255)
                                                       gTATGGCGGGGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGGGGGTGGGGAAACATTCTTTTTGGGT‑GGGGTTTACCctct          >  1:158115/1‑90 (MQ=255)
                                                               gTGGCCGCCGCAGGTTTTTCGCTGACGCTCCTTACGCTGCCGGGGCTGGGGAAACATGCTTTTTGGGTGGGCTTTAACCGCTTTTTTggg   >  1:363463/1‑89 (MQ=255)
                                                               gTGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGGGGGGGGGGAATCATGCTTTTTGGGG‑GGCCTTTAACGCCTTTTTTGCg  >  1:559219/1‑90 (MQ=255)
                                                                             |                                                                            
TATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCT‑GGCATTAACCGTCTTTCTGGCG  >  NZ_CP009273/4248825‑4248977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑