| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,248,906 | 0 | G | T | 60.0% | 11.7 / 22.9 | 15 | A395S (GCG→TCG) | dinF | MATE family efflux transporter DinF |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base T (9/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCT‑GGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCT > NZ_CP009273/4248825‑4248994 | tATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCGGGGTTTTTGCTGGCg > 2:742969/1‑90 (MQ=255) tgctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGGGGCGCCGCCGGGTTTTTGCTGACGCTcc > 1:331879/1‑90 (MQ=255) gctgGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCCCCGGGTTTTCGCTGACGCTCCt > 1:822985/1‑90 (MQ=255) aCGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGc < 1:196074/90‑1 (MQ=255) gCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCCGGGTTTTCGCTGACGCTCCCTACGCTGc > 1:655522/1‑90 (MQ=255) taGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTgg < 1:536379/90‑1 (MQ=255) taGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTgg < 2:4828/90‑1 (MQ=255) ggCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGt < 2:372799/90‑1 (MQ=255) cgcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTaa < 2:581113/90‑1 (MQ=255) gcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGGCGCCGCGGGGTTTTCGCCGGCGCCCCCTACGCTGCCGGGGCGGGGGAAt > 1:730593/1‑90 (MQ=255) gcAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGGGGGCGCCCCCGGGTTTTCGCTGACGCTCCTTTCGCCGCCGGGGCTgggggaa > 1:701091/1‑86 (MQ=255) gTATGGCGGGGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGGGGGTGGGGAAACATTCTTTTTGGGT‑GGGGTTTACCctct > 1:158115/1‑90 (MQ=255) gTGGCCGCCGCAGGTTTTTCGCTGACGCTCCTTACGCTGCCGGGGCTGGGGAAACATGCTTTTTGGGTGGGCTTTAACCGCTTTTTTggg > 1:363463/1‑89 (MQ=255) gTGGCCGCCGCAGGGTTTTCGCTGACGCTCCTTACGCTGCCGGGGGGGGGGAATCATGCTTTTTGGGG‑GGCCTTTAACGCCTTTTTTGCg > 1:559219/1‑90 (MQ=255) tGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCT‑GGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTctct < 2:504228/90‑1 (MQ=255) | TATCTGCTGGACGGCATGTTTATAGGCGCAACGCGTGCCACCGAAATGCGTAACAGTATGGCGGTGGCCGCCGCAGGTTTTGCGCTGACGCTCCTTACGCTGCCGTGGCTGGGTAATCATGCTTTGTGGCT‑GGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCT > NZ_CP009273/4248825‑4248994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |