| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 45.2% | 39.5 / 21.4 | 42 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (14/9); new base A (0/19); total (14/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAG > NZ_CP009273/4058870‑4059039 | aaTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGa < 2:742800/90‑1 (MQ=255) aTTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGaa < 2:1003630/90‑1 (MQ=255) tGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGCAAACCCGGTAAAAAACTTCCCCACC‑AGCAAGa > 2:648875/1‑90 (MQ=255) ccTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATa < 1:765201/90‑1 (MQ=255) tttAGGCCAAATTTTTTCCCGCGAGTCGAGAAAAAACCCGGTGAGCATATCGGAAAACGCGGAAAAAACCTCCCCCACA‑AAAAAGATAAt < 2:37062/90‑1 (MQ=255) ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATc > 1:1534592/1‑90 (MQ=255) ttttcCCGCGAGTCGAGAAAAAACCGGGTGAGCATATGGGAAACCGCGGAAAAAAACTCCGCCACC‑AGAAAGATAATCCCGCCATAGTaa < 1:1191541/90‑1 (MQ=255) ttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCa > 2:35632/1‑90 (MQ=255) cgcgAGTCGAAAAAAAACCGGGGGAGAAAACCGGAAAACGCGAAAAAAACCTCCCCCCCA‑AAAAAAAAAACCCCGCCATAGTAAGCAGGc < 2:924420/90‑1 (MQ=255) cgcgAGTCGAAAAAAAACCCGGTGACAATATCGAAAACCGCGGAAAAAAACTCCGCCCCC‑AAAAAGATAACCCCGCCATAGTAAGCAGGc < 1:734042/90‑1 (MQ=255) gggagtcgaaaAAAAACCCGGTGAGCATATGGGAAAACGCGAAAAAAACCTCCCCCCCA‑AAAAAAAAAACCCCGCCATAGTAAGCAGGCa < 2:1534592/88‑1 (MQ=255) gggagtcgaaaAAAAACCCGGGGAGAATATCGGTAACCGCGAAAAAAAACTTCGCCACC‑AGAAAAATAATCCCGCCATAGTAAGCAGGCa < 1:1438800/88‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGa < 1:1148711/62‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGa > 2:1148711/1‑62 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCa > 2:967829/1‑90 (MQ=255) gcgAGTCGAGAAAAAACCCGGTGAGCATATCGGAAAACGCGGAAAAAACCTCCCCCACA‑AAAAAGATAACCCCGCCATAGTAAGCAGGCa < 2:608822/90‑1 (MQ=255) gcgAGGCGAAAAAAAACCCGGTGAGCATATCGAAAAACGCGAAAAAAAACTCCGCCACC‑AAAAAGATAACCCCGCCATAGTAAGCAGGCa < 2:534704/90‑1 (MQ=21) gagtcgagtAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATc > 1:438855/1‑90 (MQ=255) aaaCCCGGTGAGCATATGGGTAAACGGGGAAAAAACCTCCCCCACA‑AAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:1477036/90‑1 (MQ=255) cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat > 1:692881/1‑90 (MQ=255) ggTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata > 2:662030/1‑90 (MQ=255) ggTGAGCATATCGGAAAACGGGGTAAAAAACTTCGCCCCC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 1:442853/90‑1 (MQ=255) ggTGAGCATAT‑GGAAAACCGGGGAAAAAACCTCCCCCCCAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 2:49707/90‑1 (MQ=255) gAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGa > 2:1126670/1‑40 (MQ=255) gAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGa < 1:1126670/40‑1 (MQ=255) tCGGTAAACGGGAAAAAAAACTTCCCCACC‑AAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:174749/90‑1 (MQ=255) tCGGAAAACGGGAAAAAAAACTCCCCCACA‑AAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:644460/90‑1 (MQ=255) cGGTAAACGCGGAAAAAAACTTCCCCACC‑AAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:105290/90‑1 (MQ=255) cGGTAAACGCGAAAAAAATCTTCGCCCCC‑AGAAAAATAAACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:68002/90‑1 (MQ=255) cGGAAAACGCGAAAAAAACCTCCCCCCCA‑AAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 1:1144636/90‑1 (MQ=255) gaaaaCGCGAAAAAAAACTTCCCCCCA‑AAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 1:1456257/88‑1 (MQ=255) aaaCGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa > 1:1262894/1‑90 (MQ=255) aaCGCGAAAAAAACCTCCCCCACA‑AAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAc < 2:692881/90‑1 (MQ=255) gggaaaaaaaCTTCCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACaga < 2:306854/86‑1 (MQ=255) ggTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt > 2:1484695/1‑90 (MQ=255) ggTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGt > 2:1243294/1‑90 (MQ=255) gTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACagctgt > 1:406768/1‑86 (MQ=255) gTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTa > 2:1442365/1‑90 (MQ=255) tcccccaca‑aAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:540884/86‑1 (MQ=255) tcccccaca‑aAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 2:1323100/86‑1 (MQ=255) ccccccaca‑aAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 2:850257/86‑1 (MQ=255) ccccaca‑aAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCagag < 2:129913/88‑1 (MQ=255) | AATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACC‑AGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAG > NZ_CP009273/4058870‑4059039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |