| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,169 | 0 | T | G | 45.2% | 36.3 / 13.9 | 31 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (1/16); new base G (14/0); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937087‑937252 | aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:4290/90‑1 (MQ=255)aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 1:270763/90‑1 (MQ=255) gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACt > 1:251125/1‑90 (MQ=255) gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCt > 1:445414/1‑90 (MQ=255) cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATc > 1:893178/1‑90 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGc < 2:814347/90‑1 (MQ=255) aaaaCAGTAAGCTGGTAGTGCTGTCTGGTAATAACCCTGGCGAAACGCGAATGTGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCAc < 1:617538/90‑1 (MQ=255) aaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACg < 2:6185/90‑1 (MQ=255) aCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTATCTTGAACAGGGGCGcc > 2:1368707/1‑90 (MQ=255) tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaa < 1:1081173/90‑1 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:1527231/61‑1 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTAc > 1:1527231/1‑61 (MQ=255) tGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAAAAGGCACGCCAGAAAACTAAAGccc > 1:365808/1‑90 (MQ=255) gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAAAAAGCACCCCAGAAAAATAATGcccc > 2:243392/1‑89 (MQ=255) tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCCGAAATCTAATGCCCGCATg > 1:448005/1‑90 (MQ=255) ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatc < 1:214190/90‑1 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAAAAAGCACGCCCGAAAAATAATGCCCGCCTGatcatcatc > 2:1114719/1‑90 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTAc > 1:1393300/1‑38 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 2:1393300/38‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 2:1333711/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 2:1447219/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc < 2:496588/90‑1 (MQ=255) cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc > 1:1475508/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCCCGCCCGAAAAATAAAGGCCGCATGGTCCTCATCGATcc > 2:1010302/1‑90 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 1:1327553/90‑1 (MQ=255) aaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCta < 1:1114719/90‑1 (MQ=255) aaCGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCta > 2:1005573/1‑90 (MQ=255) gAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg < 2:448005/90‑1 (MQ=255) gTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCCCGCCATACCCACACCg > 2:44703/1‑90 (MQ=255) ggCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAAACTAAAGCCCCCCTGATCATCATCGATCCCCGCCCTACCCACAcccgg > 2:292080/1‑87 (MQ=255) gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg < 1:31862/90‑1 (MQ=255) | AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937087‑937252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |