| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 997,447 | 0 | T | G | 61.5% | 11.3 / 24.9 | 26 | V62G (GTA→GGA) | elfG | fimbrial protein |
| Reads supporting (aligned to +/- strand): ref base T (2/8); new base G (16/0); total (18/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGC > NZ_CP009273/997358‑997532 | cGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGt < 2:725118/90‑1 (MQ=255) ggCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAg > 2:906841/1‑90 (MQ=255) actCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAAttt > 2:255570/1‑90 (MQ=255) actCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAAttt > 1:1041403/1‑90 (MQ=255) actCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGAAAAAttt > 2:283382/1‑90 (MQ=255) actCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGGTAAAGAAAAAatt > 2:1478178/1‑90 (MQ=255) aGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATc < 1:561159/90‑1 (MQ=255) aTCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGGGTTAAAGAAAAAATTTTTTAGTGGa > 2:1472844/1‑90 (MQ=255) aTCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAATTTTTTCAGTGGa > 1:1017901/1‑90 (MQ=255) aTCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAATTTTATCAGTggg > 2:1318780/1‑89 (MQ=255) aTCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAATTTTATCAGTGGa > 1:704869/1‑90 (MQ=255) aTCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAAATTTTTTAGTggg > 2:466730/1‑89 (MQ=255) tataTTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGACAAATTTTATCAGTGGAAcgaa > 1:651448/1‑90 (MQ=255) tatTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAAcgaatc < 1:1182220/90‑1 (MQ=255) ggCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAAcgaatcgaat < 2:878431/90‑1 (MQ=255) gCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATg < 1:134526/90‑1 (MQ=255) gTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGAAAAATTTTTTCAGTGGAAAGAATCGAATGat > 2:456316/1‑90 (MQ=255) gTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGGAGTTAAAGAAAAAATTTATTAGTGGAAAGAAACGAATGat > 2:1250920/1‑90 (MQ=255) aTTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGattatt < 1:487605/90‑1 (MQ=255) ccTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGGACGAATCGAATGATTATTATGTTTCCTGTGATTg < 1:1318780/90‑1 (MQ=255) ccTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTg < 1:1472721/90‑1 (MQ=255) aagaagCAAATACGGCAGGGGGAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAg > 2:441606/1‑90 (MQ=255) aagaagCAAATACGGCAGGGGGAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAg > 1:1221679/1‑90 (MQ=255) agaagCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGa > 1:1333246/1‑90 (MQ=255) aagCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACa < 2:651448/90‑1 (MQ=255) ggCAGGGGGAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAg > 2:1137987/1‑90 (MQ=255) ggggGAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAAACAATGTCAGAAGTGGc > 2:183382/1‑90 (MQ=255) | CGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGC > NZ_CP009273/997358‑997532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |