| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 66.7% | 9.8 / 24.6 | 27 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (18/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152641‑1152816 | gATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGaaa > 1:630500/1‑90 (MQ=255) cTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATACTg > 1:1514740/1‑90 (MQ=255) tGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGAt < 1:562217/90‑1 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGTGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGttt > 1:6578/1‑90 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGATTTCGGGttt > 2:236186/1‑90 (MQ=255) gTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCttt < 1:1107862/90‑1 (MQ=255) cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTTTATTTTCttttttc > 2:370816/1‑87 (MQ=255) ttGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGGTCTCGGGTTTTAAATTTCCTTTTTCCGCAt > 1:569143/1‑90 (MQ=255) gTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGa < 1:1551670/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCt < 2:97983/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCt < 2:719586/90‑1 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATTGATCTCGGGTTTTAAATCTCCTTTTTCCGCCTTTTGGCCTTCCCTaa > 1:1024081/1‑90 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATGGGGTTTGGCGTTTAAATCTTCTTTTTTGGCATTTTGGCCTTTTGTAAAGc > 2:1312807/1‑90 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGGCCCTCCCTAAAGc > 2:352733/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGATTTTAAATCTCCTTTTTCCGCATTGTGACCTTCCCTAATGCgg > 1:17345/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATATTTGATTTCGGGGTTTTAATTTCCTTTTTCCGCATTGTGGCCTTTCCTAATGCgg > 2:101488/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGATTTTACATCTCCTTTTTCGGCATTTTGACCCTCCCTAAAGCGGGGc > 1:269389/1‑90 (MQ=255) gagGACAGAGAAACGGCGGGGAAAATACTTGGTCTCGGGTTTTAAATCTCCTTTTTCCGCCTTGTGACCTTCCCTAATGCGGGGCAAccg > 1:1327274/1‑88 (MQ=255) ggACAGAGAAACGGCGGGGAAATTACTTGGTCTCGGGTTTTATATCTCCTTTTTCCGCATTTTGGGCTTTCGTAAATCGGGGCAACTgcg > 1:1524604/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTAAATCTCCTTTTTCGGCATTGTGACCTTCCCTAAAGCGGGGGAACTgcg > 1:423199/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTAAATCTCCTTTTTCCGCATTTTGACCTTCCCTAATGCGGGGGAACcgcg > 1:1434193/1‑90 (MQ=255) gACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc < 1:972123/90‑1 (MQ=255) gACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc < 1:370816/90‑1 (MQ=255) cAGAGAAACGGCGGGGAAAATAATTGGTTTTGGGTTTTTTATCTCCTTTTTCTGGCTTGTGGACTTTCGTAATGGGGGGGAACTGcccgg > 2:972123/1‑89 (MQ=255) gagaAACGGCGGGGAAAATATTTGATCTCGGGTTTTTAAACTCCCTTTTCGGCATTGTGACCTTCCCTTATTCGGGGCAACTGCGCGAtg > 2:958260/1‑90 (MQ=255) gagaAACGGCGGGGAAAATACTGGGTCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGGCCTTCCCTAATGCGGGGGAACTGCGCGGtg > 1:1209587/1‑90 (MQ=255) cggcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTgcgc < 2:1434193/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:768491/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:17345/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:1312807/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:788304/90‑1 (MQ=255) | GATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152641‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |