| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,216,154 | 0 | T | G | 62.5% | 6.2 / 13.1 | 16 | V164G (GTG→GGG) | dld | D‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216076‑2216236 | cGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGcc < 2:119101/90‑1 (MQ=255) tGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGGATTTTTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCGGCGcata > 2:544392/1‑90 (MQ=255) atcatcGTGTATAGGCGCATCGGTCATCGGCGGGATTTGTAAAAACCCCCGCGGCCCGCTGGGGCAAAATGGCCCGGCGCATACCGaaaa > 1:141586/1‑89 (MQ=255) gtATAGGCGCATCGGTCATCGGCGGGATTTTTAAAAAAACCGGCGGCTCCCCGGGGGAACGGGGGCCCGGGGAAACCGAAAAGTCGTTAt > 1:234504/1‑90 (MQ=255) ggCGCATCGGTCATCGGCGGTATTTTTAACAAATCCGGCGGCTCGCTGGGGCAACGTGGCCCGGGGGATACCGAAATGTCGGTATTcgcg > 2:396565/1‑90 (MQ=255) ggCGCATCGGTCATCGGCGGTATTTGTAACAACCCCGGCGGCTCGCTGGGGCAACGTGGCCCCGCGGATACCGAAATGTCGTTATTtgcg > 2:391188/1‑90 (MQ=255) gcgcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGGGGCCCGGCGGATACCGAAATGTCGGTATTtgcgc > 2:478350/1‑90 (MQ=255) gcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGt > 2:227648/1‑90 (MQ=255) gcATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGGGCAACGGGGCCCGGCGGATACCGAAATGTCGGTTTTTGCGCGt > 2:53888/1‑90 (MQ=255) gcATCGGTCATCGGCGGGATTTTTAAAAACTCCGGCGGCGCGCTGGGGCGAAACGGCCCGGCGGATACCGAAAAAGCGTTATTtgcgcgg > 1:500139/1‑89 (MQ=255) aTCGGTCATCGGCGGGATTTTTAAAAACTCCGGCGGGTCGCCGGGGGGACGGGGGCCGGCGTGTAACGGAATGTCGTTTTTtgcgcggtt > 1:350051/1‑87 (MQ=255) cggcggTATTTGTAACAGCTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGa < 1:269023/90‑1 (MQ=255) acTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGtat > 1:453276/1‑38 (MQ=255) acTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGtat < 2:453276/38‑1 (MQ=255) tCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTg < 1:391188/90‑1 (MQ=255) tCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTg < 2:234504/90‑1 (MQ=255) | CGCACTCAGTGATTGGATCATCGTGTATAGGCGCATCGGTCATCGGCGGTATTTGTAACAACTCCGGCGGCTCGCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTG > NZ_CP009273/2216076‑2216236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |