Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,336,7780TA54.5% 3.0 / 12.9 11M291L (ATG→TTG) yfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
Reads supporting (aligned to +/- strand):  ref base T (5/0);  new base A (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCT  >  NZ_CP009273/2336692‑2336851
                                                                                      |                                                                         
aaCAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATaaa                                                                        >  2:160922/1‑90 (MQ=255)
                   gTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaa                                                     >  1:530694/1‑90 (MQ=255)
                         ccAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCa                                               >  2:523665/1‑90 (MQ=255)
                         ccAAAAACCAGCTTTTTCCCGCCGAAAATAAAAAAGGAAACTTCCCTAACGCCGAGATACAAAAACCCCCCCGCCGCAGAGGAAGGACCa                                               <  1:352619/90‑1 (MQ=255)
                          cAATACCCGGCTTTTTCCCGGCGGCAACAAAAAAGGTAACTCCACTTAAGCCGAGATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCAt                                              <  1:348093/90‑1 (MQ=255)
                          cAAAAACCAGCTTTTTCCCGCCGGAAATATAAAAGGTAACTTCCCTTAAGCCGAGATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCAt                                              <  2:554306/90‑1 (MQ=255)
                             tAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCAtctc                                           >  1:479015/1‑90 (MQ=255)
                                   gCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGc                                     >  1:577165/1‑90 (MQ=255)
                                                             gaaaCTTCACTAAACCCGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACt           <  2:169008/88‑1 (MQ=255)
                                                                  ttCACTAAAGCGGAGATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAacac      <  2:131900/90‑1 (MQ=255)
                                                                      ctaaaGCCGAGATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCt  <  2:530694/87‑1 (MQ=255)
                                                                                      |                                                                         
AACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGGCAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCT  >  NZ_CP009273/2336692‑2336851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑