Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3620CT53.8% ‑2.6 / 14.5 13intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base C (0/6);  new base T (7/0);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 5.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGC  >  NZ_CP009273/2380277‑2380435
                                                                                       |                                                                         
tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GGCCAGAAAAAACGACATGCACATTTTT‑TTTTTCCTc                                                                       >  2:369395/1‑90 (MQ=255)
   tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATc                                                                    <  1:240122/90‑1 (MQ=255)
     ttATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GGCCTGAAAAAAAGACATGCACATTTTA‑TTTTTCCTCctctt                                                                  >  2:19402/1‑89 (MQ=255)
       ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GGCCAGAAAAAAAGACAAGCACATTTTT‑TTTTTCCTCTTCTGtt                                                                >  2:451248/1‑90 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGTATCTGGAAAAAG‑GAAAAACACATTTTT‑TTTTTCCTCTCATGttttgtt                                                           >  1:535217/1‑87 (MQ=255)
            tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GGCCAGAAAAAACGACATGCACATTTTT‑TTTTTCCTCCTCtttttttaa                                                           >  2:305690/1‑87 (MQ=255)
                  ataCAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGa                                                     <  1:484276/90‑1 (MQ=255)
                            aaaTAATAAAAATTTCTCGAGGCGG‑GTGCAGAAAAAAAGACAAGCACATTTTT‑TTTTTTCTCATCTGTTTTTAGATGGGAAACAAGAAAt                                           >  1:363257/1‑90 (MQ=255)
                               taataaAAATTTCTCGAGGCGG‑GGCCAGAAAAAACGACATGCACAATTTT‑TTTTTCCTCCTCTTTTTTTAAAAGCGAAAAAAGaaaatta                                        >  2:166342/1‑86 (MQ=255)
                                    aaaaTTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTATTTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgg                                    <  1:90517/90‑1 (MQ=255)
                                       aTTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga                                <  2:360570/90‑1 (MQ=255)
                                                   gg‑gTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATggg                    <  2:136296/90‑1 (MQ=255)
                                                                      aTGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGc  <  2:460564/90‑1 (MQ=255)
                                                                                       |                                                                         
TGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGG‑GTCCAGAAAAAGCGACATGCACATTTTA‑TTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGC  >  NZ_CP009273/2380277‑2380435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑