| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,805 | 0 | T | G | 60.0% | 8.4 / 13.0 | 16 | V785G (GTT→GGT) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (9/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATG > NZ_CP009273/2852725‑2852879 | tATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGGGTGCCAAAAGAGGGTATTAAacg > 2:404146/1‑90 (MQ=255) aGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGGGGGGCAAAAGAGGGTATTAAGCGCGCACgg > 1:393882/1‑90 (MQ=255) aGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACgg > 2:126914/1‑90 (MQ=255) aGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGGTGCAGCTCTGGCAGGCGTGGCAAAAGAGGATATTAAGCGCGCACgg > 2:320470/1‑90 (MQ=255) gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGGGTGCCAAAAGAGGGTATTAAGCGCGCACGGc > 2:211867/1‑90 (MQ=255) gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCGCTGGCCGGGGGGCCAAAAAAGGGTATTAAACGCGCCCGGc > 1:574998/1‑90 (MQ=255) gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGGTGCAGCCCTGGGAGGCGGGGCAAAAGAGGGTATTAAACGCGCACGGc > 1:113791/1‑90 (MQ=255) tGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCg < 1:95740/90‑1 (MQ=255) tGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCCCGGCAAAAACTGCg > 2:121683/1‑90 (MQ=255) tGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCACGGCAAAAACTGCg > 2:85372/1‑90 (MQ=255) gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGGGCCAAAAGAGGGTATTAAACGCGCACGGCAAAAGCTGCggg > 1:571223/1‑88 (MQ=255) cTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAAc < 2:571223/90‑1 (MQ=255) ggCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGGTATTAAGCGCGCCCGGCAAAAGCTGCGTGAGGGGGAAAGCATTTTGCCGAAcgc > 1:477872/1‑90 (MQ=255) ctctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGc < 1:447423/90‑1 (MQ=255) aGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGa > 1:196461/1‑90 (MQ=255) gCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATg > 1:554003/1‑90 (MQ=255) | TATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATG > NZ_CP009273/2852725‑2852879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |