| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,594,920 | 0 | A | C | 64.3% | 8.9 / 12.5 | 14 | V176G (GTG→GGG) | ftsX | permease‑like cell division protein FtsX |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/9); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCC > NZ_CP009273/3594833‑3595000 | cGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATcacc > 2:127185/1‑90 (MQ=255) cGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCCCCACCGCCa < 2:324135/90‑1 (MQ=255) gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt > 1:291852/1‑90 (MQ=255) gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt > 1:92805/1‑90 (MQ=255) gCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtttt > 2:441408/1‑90 (MQ=255) ccccgcgGCTTATTCAGTGTTCCCGCCCCCGGA‑AAATGGAGTTTGGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata < 1:441408/87‑1 (MQ=255) gTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGca > 2:309199/1‑90 (MQ=255) tATTCAGTGATCCCGCCCCCTGA‑AAATCGATTTTCGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac < 1:127185/90‑1 (MQ=255) aTTCAGTGATTCCGCCCCCTGGAAAATCGATTTTCGGGACCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcac < 2:511528/90‑1 (MQ=255) aGTGCTCCCGCCCCCTGG‑AAATGGATTTTCGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCAccacca < 1:109169/90‑1 (MQ=255) cTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGtt > 2:504962/1‑90 (MQ=255) gaaaaCCGAGTTCCGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTAc < 1:365285/88‑1 (MQ=255) gAGTTTCGGAACCCCCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTc < 1:286974/90‑1 (MQ=255) ttCGGGACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAccc < 2:153140/90‑1 (MQ=255) ttCGGAACCCCCCCCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAccc < 1:431163/90‑1 (MQ=255) | CGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGG‑AAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCC > NZ_CP009273/3594833‑3595000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |