| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 6,981 | 0 | A | G | 54.5% | 4.9 / 10.6 | 11 | Y327H (TAC→CAC) | yaaJ | sodium:alanine symporter family protein |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base G (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATC > NZ_CP009273/6898‑7063 | aaGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTtgt > 1:82466/1‑90 (MQ=255)aaGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTtgt > 2:581176/1‑90 (MQ=255) ggTAACAAACTCAACACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGcc < 1:334782/90‑1 (MQ=255) tAGCCCCCGCCCCCCTTTCGGAATAAGCTAAATCCCTTCCAGGGGCATGGGGGTTGTGCCTTCCCCCGCCAGTAATATCAGCATGGCGCt < 1:524271/90‑1 (MQ=255) aGCCCCCGCATCCCCTTCTGAAAAAGCTAAATCCCTCCAGGGGGCTTGTGGGTTGGGCCGTTCCCCGCCAGTAATATCAGCATGGCGCtt < 2:241911/90‑1 (MQ=255) cccccGCACCGCTTTCTGAATAAGCTAAATCCCTCCCGGCGGCATGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGc < 2:94585/88‑1 (MQ=255) cccGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCg > 1:141696/1‑90 (MQ=255) aaaaaGCTAAATCCCTCCGAGGGGAATGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGgt < 1:412356/87‑1 (MQ=255) aaTCCCTCCCAGCGGCATGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaa < 2:222990/90‑1 (MQ=255) ttCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGcc > 1:130715/1‑90 (MQ=255) ccAGGGGTATGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 2:371655/90‑1 (MQ=255) ggtttgtgGGTTGTGCCTTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAatcatc < 2:141696/86‑1 (MQ=255) | AAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATC > NZ_CP009273/6898‑7063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |