| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 60.0% | ‑5.4 / 11.7 | 15 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/1); new base A (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058879‑4059038 | ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATc > 2:443153/1‑90 (MQ=255) tttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACAAAAAAAATAACCCCGCCATAGTAAGc < 2:96318/90‑1 (MQ=255) ttcccGGGAGTCGAAAAAAAACCCGGGGAACATATGGGAAACCGCGAAAAAAACCTCCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGc < 1:346126/87‑1 (MQ=255) gcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCa > 1:116613/1‑90 (MQ=255) gcgAGTCGAGAAAAAATCCGGTGAGCATATCGGAAAACGGGGAAAAAAACTCCCCCCCCAAAAAGATAACCCCGCCATAGTAAGCAGGCa < 2:560406/90‑1 (MQ=255) gcgAGTCGAGAAAAAATCCGGGGAGCATATCGGAAAACGCGGTAAAAACCTCCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCa < 2:579294/90‑1 (MQ=255) aacgccGATGACCATACGGGTAAACGAGAAAAAAACCTCCCCCCCAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 1:447826/86‑1 (MQ=255) aaaTCCGGGGAGCATATCGGAAAACGCGGAAAAAAACTTCCCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:501502/90‑1 (MQ=255) actCCGGTGAGAATATCGGAAACGGCGGAAAAAAACTTCCCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg < 2:39311/88‑1 (MQ=255) tCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt > 2:146204/1‑90 (MQ=255) tCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt > 2:580307/1‑90 (MQ=255) tatCGGAAAACCCGGAAAAAAACTTCCCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTga < 2:170040/90‑1 (MQ=255) ggaaaaaCGCGAAAAAAAACTCCCCCCCAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagga < 2:327757/86‑1 (MQ=255) tccGCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:443153/88‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:114105/1‑90 (MQ=255) | TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058879‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |