Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,098,3370TG58.3% 7.6 / 10.3 12G286G (GGT→GGG) pfkA6‑phosphofructokinase
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACT  >  NZ_CP009273/4098252‑4098413
                                                                                     |                                                                            
tGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGCGTGGTGTTGGGAttc                                                                          >  1:186890/1‑88 (MQ=255)
           cGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGCGTTTTGTAGGGATCCCGAAAGAAAAg                                                               >  2:48309/1‑90 (MQ=255)
                   ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGGCGTTGTGTTGGGATTCAGAAAGAACAGCTGGttta                                                       >  1:337079/1‑88 (MQ=255)
                     cTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcacc                                                     <  1:48309/90‑1 (MQ=255)
                      ttCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGGTGTGTGGGGATCCCGAACGAACAACCGGGTcacca                                                    >  1:291511/1‑90 (MQ=255)
                       tCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTGGGGATCCAAAAAGAACAGCCGGGTtacccc                                                   >  2:526900/1‑88 (MQ=255)
                                                    gctgctgGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAAAATGaa                      >  2:237821/1‑90 (MQ=255)
                                                    gctgctgGCAGGTTACGGCGGTCGGTGTGTGGGGATCCAAAAAGAACAACGGGGTTACCACGGCAACATTGACGCGGTGGAAAAAAAGaa                      >  1:152205/1‑90 (MQ=255)
                                                         tgGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTc                 <  2:273809/90‑1 (MQ=255)
                                                                 tACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCaaa         <  2:367566/90‑1 (MQ=255)
                                                                    ggcggTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACCACATCATCCACGCTATCGAAAACATGAAGCGTCCGTTCAAAggg      >  2:252301/1‑89 (MQ=255)
                                                                        gTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCTGTTCAAAGGTGAct  <  1:244842/90‑1 (MQ=255)
                                                                                     |                                                                            
TGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACT  >  NZ_CP009273/4098252‑4098413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑