Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273381,2530TG61.5% 3.9 / 11.9 13V189G (GTG→GGG) tauAtaurine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (8/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 6.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAAC  >  NZ_CP009273/381190‑381336
                                                               |                                                                                   
gagaTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGTCgggg                                                           >  2:314206/1‑89 (MQ=255)
  gaTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAAAGCCCTGGAAAAAAACGGCAAGGGGTTGACCGCTTCTGTACAAGGCggggcg                                                         >  2:264767/1‑87 (MQ=255)
                 tATGTCTGGGCACCGGCGGATAACGCCCCTGGAAAAGACGGCCAGGGGGTGTGCGATTTTGAACAGGTTGGGGCGGGGGGGCCGCCaaag                                          >  1:179649/1‑88 (MQ=255)
                      cTGGGCACCGGCGGGTAAAGCCCCGGAAAAAAACGGCAAGGGGTTGGCCGGTTCTGGACAGGTCGGGGCGGGGGGGGGGCCCAAGCTggg                                     >  1:493905/1‑89 (MQ=255)
                       tGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGAc                                    <  2:582254/90‑1 (MQ=255)
                        gggCACCGGCGGTTAACGCCCTGGGAAAAGACGGCAAGGGGTTGACCGATTCTGAACAGGGCGGGCGGTGGGGGGCGCCAACGCTGGGCg                                   >  2:212704/1‑90 (MQ=255)
                        gggCACCGGCGGTTAACGCCCTGGAAAAAAACGGCAAGGGGTTGACCGATTCTGAACAGGGCGGGGAGGGGGGGGCGCCAACGCTGGGCg                                   >  2:171062/1‑90 (MQ=255)
                        gggCACCGGCGGTTAAAGCCCCGGAAAAAAACGGCAAGGGGTTTGCCGATTCTTAACAGGGGGGGCAGGGGGGGGCGCCCACCCTGGGCg                                   >  1:582254/1‑90 (MQ=255)
                              cggcggTTAACGCCCCGGAAAAAAACGGCAAGGGGGTGACCGGTTCTGAACAGGGCGGGGAGGGGGGGGCGCCAACGCTGGGCGTCTggg                             >  1:530614/1‑90 (MQ=255)
                                    ttAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTgc                       <  1:192584/90‑1 (MQ=255)
                                           ccTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGa                >  2:178483/1‑90 (MQ=255)
                                              ggAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAttt             <  1:236656/90‑1 (MQ=255)
                                                         gCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAAc  <  1:264767/90‑1 (MQ=255)
                                                               |                                                                                   
GAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAAC  >  NZ_CP009273/381190‑381336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑