| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 54.5% | 8.6 / 12.6 | 11 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG > NZ_CP009273/535928‑536099 | gcCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgggtg > 2:414228/1‑90 (MQ=255) tAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTc < 1:522735/90‑1 (MQ=255) aTCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTTTTTTTAATTTTTCAGTCAGCcg > 1:569428/1‑90 (MQ=255) tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTAAGGGGGGGTTTTGTTTAATTTTTCGGTGAGcccc > 1:507632/1‑88 (MQ=255) gTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCGTTCTCCGGGTAAGGGGGGGTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGc > 2:3251/1‑90 (MQ=255) aaTCTGGCCCCTGCAATGCCCCTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCt > 2:359075/1‑90 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCCGGTAAGGGGGGGTTTTGTTTAATTTTTTAGTCAGCCGCGGAAGACCGTTAt > 1:372368/1‑90 (MQ=255) aTGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGGTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCa > 2:70955/1‑90 (MQ=255) tCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGttt < 2:507632/90‑1 (MQ=255) tCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGttt < 2:569428/90‑1 (MQ=255) tAAGGTGTGTGTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGtt > 2:331167/1‑90 (MQ=255) aaGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTtg < 2:468642/90‑1 (MQ=255) | GCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTG > NZ_CP009273/535928‑536099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |