| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 546,710 | 0 | T | G | 53.8% | 6.8 / 13.6 | 13 | G272G (GGT→GGG) | ybcF | carbamate kinase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (7/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCAT > NZ_CP009273/546626‑546798 | ggATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGGGGGTATTTTAGAAGCCGGGGTaaaac > 2:485538/1‑88 (MQ=255) gAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCcgcg < 1:311906/90‑1 (MQ=255) gTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGGGGGGGTTTTTCAGAAGCCGCGGGAAAACcccggg > 2:311906/1‑88 (MQ=255) tAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGGGGGGGGGTATGTGAGAAGCCGAGGGAAAACCCCggggg > 2:80651/1‑89 (MQ=255) gcgcCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAtt < 2:195320/90‑1 (MQ=255) aTTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGGGGGTATGTCAGAAACCGGGGGAAACCCCCGtggggtggggg > 2:373342/1‑89 (MQ=255) cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAAt < 1:399404/90‑1 (MQ=255) ttCGATGGGGCCGAATGTAACGGCGGTGAGGGGGTATGTCAGAAACCGTGGGAAAACCCCGGGGGTTGGGGGGGTTTTGCGAATTTAaga > 2:555554/1‑90 (MQ=255) cGATGGGGCCGAATGTAACGGCGGTGGGGGGGTTTGTTAGAAGCCGGGGGAAAACCCCGGGGGTTGGGGGGGTGTTGCGAATTTAagagg > 1:452006/1‑89 (MQ=255) cGATGGGGCCGAATGTAACGGCGGTGAGGGGGTTTGTCGGAAGCCGGGGGGAAACCCCGGGGGGTGGGGGGGGTTTGCGAATTGAAgaga > 1:534325/1‑90 (MQ=255) aTGGGGCCGAATGTAACGGCGGTGGGTGGGTATGTCAGAAGCCGGGGGAAACCCGCGGGGGTTGGGGGGTTATTGCGGATTGgagagaag > 2:18494/1‑88 (MQ=255) gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGGAGGGGGGGGCCGTATTTTGCTGTAGTCGTTgg > 1:380174/1‑90 (MQ=255) gTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCAt < 1:250643/90‑1 (MQ=255) | GGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCAT > NZ_CP009273/546626‑546798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |