| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,356,378 | (A)8→7 | coding (213/2382 nt) | yhcD → | fimbrial biogenesis outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,356,371 | 0 | A | . | 96.5% | 221.9 / 2.1 | 58 | coding (206/2382 nt) | yhcD | fimbrial biogenesis outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base . (46/10); total (48/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01 | |||||||||||
GTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAA > NZ_CP009273/3356284‑3356455 | gtgtAGATCCTAATATATCGCATTATTTTTCCCGTTCAACCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGaaa > 2:461924/1‑90 (MQ=255) tAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGaaaaaa < 2:318370/90‑1 (MQ=255) aGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGaaaaaaa > 2:61586/1‑90 (MQ=255) cTAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCaa > 2:79598/1‑90 (MQ=255) tAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAAc > 2:915292/1‑90 (MQ=255) tAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAAc > 1:459539/1‑90 (MQ=255) tAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAAc > 1:532268/1‑90 (MQ=255) atatatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACAt < 2:829429/90‑1 (MQ=255) atatatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACAt > 1:737554/1‑90 (MQ=255) tatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAAc < 1:386286/85‑1 (MQ=255) tatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAAc > 2:386286/1‑85 (MQ=255) tatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGc > 2:41731/1‑90 (MQ=255) tatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGc > 1:320980/1‑90 (MQ=255) tatCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGc > 2:15394/1‑90 (MQ=255) atCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCt > 1:252375/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:232007/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:297905/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:262485/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:917398/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 1:830070/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa < 1:517775/90‑1 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:559225/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 2:770527/1‑90 (MQ=255) tCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTa > 1:388672/1‑90 (MQ=255) gCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTAcg > 1:746805/1‑90 (MQ=255) gCATTATTTTTCCCGTCCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTAcg > 2:524255/1‑90 (MQ=255) attTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCtt < 2:513683/90‑1 (MQ=255) attTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCtt > 2:692403/1‑90 (MQ=255) attTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCtt < 1:232865/90‑1 (MQ=255) tCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTtgatg > 2:437212/1‑90 (MQ=255) cGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGaaa > 1:682051/1‑90 (MQ=255) ttCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAAt > 2:112546/1‑90 (MQ=255) gCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCg > 2:81728/1‑90 (MQ=255) ttttttGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAtt > 1:431691/1‑90 (MQ=255) ttttttGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAtt < 2:362845/90‑1 (MQ=255) ttttttGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAtt < 1:614410/90‑1 (MQ=255) ttttttGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACAtt < 2:508286/90‑1 (MQ=255) aGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGAt > 1:773432/1‑90 (MQ=255) aGGTGAATATTCACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGAt > 1:45114/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTc > 2:620000/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 1:594855/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 1:239391/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 2:8807/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 1:388260/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 1:436270/1‑90 (MQ=255) cACTGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCt > 1:856747/1‑90 (MQ=255) tGATAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGca > 2:738893/1‑90 (MQ=255) aTAGTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGcaac > 1:680691/1‑90 (MQ=255) gTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAg > 2:399675/1‑90 (MQ=255) gTATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAg > 1:366880/1‑90 (MQ=255) tATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTc < 2:424188/65‑1 (MQ=255) tATCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTc > 1:424188/1‑65 (MQ=255) aTCAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGcc < 2:12229/90‑1 (MQ=255) cAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCg > 1:724258/1‑90 (MQ=255) cAGTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCgg > 1:565378/1‑90 (MQ=255) gTAAATGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGtt > 2:189686/1‑90 (MQ=255) aTGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTaaa > 1:504158/1‑90 (MQ=255) aTGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTaaa > 1:674431/1‑90 (MQ=255) aTGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTaaa > 1:717026/1‑90 (MQ=255) aTGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTaaa > 2:180715/1‑90 (MQ=255) aTGGCG‑AAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTaaa > 1:157084/1‑90 (MQ=255) | GTGTAGATCCTAATATATCGCATTATTTTTCCCGTTCAGCCCGTTTTTTGCCAGGTGAATATTCACTGATAGTATCAGTAAATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAA > NZ_CP009273/3356284‑3356455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |