| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,681,554 | (T)5→4 | coding (980/3474 nt) | bcsC ← | cellulose synthase complex outer membrane protein BcsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,681,550 | 0 | T | . | 96.7% | 113.0 / ‑2.1 | 30 | coding (984/3474 nt) | bcsC | cellulose synthase complex outer membrane protein BcsC |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (14/15); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GCTGGATCGCCAGCCAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGTC > NZ_CP009273/3681465‑3681630 | gctgGATCGCCAGCCAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCttttc < 2:456136/90‑2 (MQ=255) aTCGCCAGCCAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGAt > 2:798955/1‑90 (MQ=255) cGCCAGCCAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTc < 2:183144/90‑1 (MQ=255) cagccagTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGcc > 1:225303/1‑90 (MQ=255) cGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTgg < 1:776988/90‑1 (MQ=255) aCTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGAt < 2:603335/90‑1 (MQ=255) tttCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCg < 2:809285/90‑1 (MQ=255) agcagACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCtt < 1:98605/90‑1 (MQ=255) gACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTg < 1:557959/90‑1 (MQ=255) gACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTg > 2:404637/1‑90 (MQ=255) gACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTg < 2:225303/90‑1 (MQ=255) gTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGaa > 2:371677/1‑90 (MQ=255) ttCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCg < 2:261490/77‑1 (MQ=255) ttCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCg > 1:261490/1‑77 (MQ=255) ttCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAAt > 1:911054/1‑90 (MQ=255) ttCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAAt < 2:723024/90‑1 (MQ=255) ccATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATa < 2:275766/90‑1 (MQ=255) tGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTg > 2:284716/1‑78 (MQ=255) tGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTg < 1:284716/78‑1 (MQ=255) tCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGc > 2:334830/1‑90 (MQ=255) tCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCCCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGc > 2:447484/1‑90 (MQ=255) ttgttgCTGCTGTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCa < 2:240237/90‑1 (MQ=255) gctgTGCGGGTCCAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGccccc < 2:253940/90‑1 (MQ=255) ccAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACt < 2:366992/90‑1 (MQ=255) ccAGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACt < 1:61207/90‑1 (MQ=255) aGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGccccc > 1:879222/1‑77 (MQ=255) aGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGccccc < 2:879222/77‑1 (MQ=255) aGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGt > 2:211339/1‑90 (MQ=255) aGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGt > 2:686116/1‑90 (MQ=255) aGTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGt > 2:859493/1‑90 (MQ=255) gTGCGAGGGC‑TTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGTc > 2:791109/1‑90 (MQ=255) | GCTGGATCGCCAGCCAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGTC > NZ_CP009273/3681465‑3681630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |