Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,798,416 (T)8→7 coding (61/936 nt) rfaS ← LPS core biosynthesis protein RfaS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,798,4090T.100.0% 57.9 / NA 15coding (68/936 nt)rfaSLPS core biosynthesis protein RfaS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/8);  total (7/8)

ATTATCCATCCCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAG  >  NZ_CP009273/3798330‑3798493
                                                                               |                                                                                    
aTTATCCATCCCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGt                                                                          <  2:651835/90‑1 (MQ=255)
   atccatccCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTAc                                                                        <  2:695510/90‑1 (MQ=255)
     ccatccCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTg                                                                      <  2:332891/90‑1 (MQ=255)
        tccCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTGAAt                                                                   >  1:499/1‑90 (MQ=255)
        tccCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTGAAt                                                                   >  2:806513/1‑90 (MQ=255)
                        ttttttATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATaa                                                   <  2:177297/90‑1 (MQ=255)
                             tattaATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGc                                              <  1:371446/90‑1 (MQ=255)
                                               tttttGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTataaaata                            >  2:336252/1‑90 (MQ=255)
                                                               atTTTTTATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAag            >  2:281385/1‑90 (MQ=255)
                                                                 tttttATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAagtt          <  2:55143/90‑1 (MQ=255)
                                                                  ttttATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAagtta         <  1:433470/90‑1 (MQ=255)
                                                                  ttttATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAagtta         <  2:499/90‑1 (MQ=255)
                                                                    ttATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAagttagt       >  1:466109/1‑90 (MQ=255)
                                                                    ttATCTTGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAagttagt       >  2:247014/1‑90 (MQ=255)
                                                                         ttGTACTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAg  >  2:237794/1‑90 (MQ=255)
                                                                               |                                                                                    
ATTATCCATCCCTATTTTTGATATTTTTTTATTAATCCAGTTATATTTTTTTGGTGTTGTTATATTTTTTATCTTGTACTTTTTTTTCAGGTACTGAATCATTTTTAATTCATAATCTGCAACCCAATTTATAAAATAAATAGTCATAATAAAGTTAGTTCCAG  >  NZ_CP009273/3798330‑3798493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: