Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,165,187	(A)5→4	intergenic (‑233/‑129)	coaA ← / → BW25113_RS20630	type I pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,165,183	0	A	.	100.0%	148.4 / NA	36	intergenic (‑229/‑133)	coaA/BW25113_RS20630	type I pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (23/13);  total (23/13)

AAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCA  >  NZ_CP009273/4165105‑4165263                                                                               |                                                                                 aaGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTa                                                                      <  1:196469/90‑1 (MQ=255)     aaTGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCt                                                                  >  2:65951/1‑90 (MQ=255)             aGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCg                                                          >  2:730772/1‑90 (MQ=255)               atatTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGtt                                                        >  1:719778/1‑90 (MQ=255)                tatTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTc                                                       >  2:509154/1‑90 (MQ=255)                tatTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTc                                                       >  1:422044/1‑90 (MQ=255)                tatTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTc                                                       >  1:671982/1‑90 (MQ=255)                  ttCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCgg                                                     >  2:278453/1‑90 (MQ=255)                      aTCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGt                                                 >  1:285482/1‑90 (MQ=255)                        cTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGttt                                               >  1:286608/1‑90 (MQ=255)                        cTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGttt                                               >  1:499425/1‑90 (MQ=255)                           aTAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTttctt                                            >  1:404877/1‑90 (MQ=255)                                    aTTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTc                                   >  1:517941/1‑90 (MQ=255)                                    aTTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTc                                   >  1:893131/1‑90 (MQ=255)                                    aTTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTc                                   >  1:849181/1‑90 (MQ=255)                                    aTTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATAGTGTc                                   <  2:600773/90‑1 (MQ=255)                                     ttATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCg                                  >  2:55252/1‑90 (MQ=255)                                        tGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCAt                               <  1:65951/90‑1 (MQ=255)                                        tGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCAt                               <  1:342271/90‑1 (MQ=255)                                         ggTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATa                              <  1:39400/90‑1 (MQ=255)                                         ggTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATa                              <  2:404877/90‑1 (MQ=255)                                            ttAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATaaaa                           >  1:60872/1‑90 (MQ=255)                                            ttAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATaaaa                           >  2:304781/1‑90 (MQ=255)                                            ttAGCAGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATaaaa                           >  2:460572/1‑90 (MQ=255)                                                 aGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGa                      <  1:144918/90‑1 (MQ=255)                                                 aGGAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGa                      <  2:539869/90‑1 (MQ=255)                                                   gAGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGAcc                    >  1:830621/1‑90 (MQ=255)                                                    aGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTc                                   >  1:672542/1‑74 (MQ=255)                                                    aGCGCATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTc                                   <  2:672542/74‑1 (MQ=255)                                                       gcATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGccc                                                              >  1:688796/1‑44 (MQ=38)                                                       gcATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGccc                                                              <  2:688796/44‑1 (MQ=38)                                                       gcATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCt                                                   <  1:178259/55‑1 (MQ=255)                                                       gcATTGTTGAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCt                                                   >  2:178259/1‑55 (MQ=255)                                                          ttgttgAGCACAATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATaaaa             <  2:35503/90‑1 (MQ=255)                                                                    caATGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGc   >  1:504887/1‑90 (MQ=255)                                                                     aaTGATGTTG‑AAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCa  <  1:304781/90‑1 (MQ=255)                                                                               |                                                                                 AAGCAATGTGACAGATATTCCATCTGATAAAAAGAATTATGGTTTAGCAGGAGCGCATTGTTGAGCACAATGATGTTGAAAAAGTGTGCTAATCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCA  >  NZ_CP009273/4165105‑4165263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑