Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,760,505	(T)5→4	coding (2438/3057 nt)	ydiJ ←	FAD‑binding and (Fe‑S)‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,760,501	0	T	.	100.0%	109.1 / NA	27	coding (2442/3057 nt)	ydiJ	FAD‑binding and (Fe‑S)‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (11/16);  total (11/16)

AAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGT  >  NZ_CP009273/1760423‑1760581                                                                               |                                                                                   aaaaCGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGa                                                                        <  2:873600/90‑1 (MQ=255)  aaaCGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGAc                                                                       <  1:566653/90‑1 (MQ=255)  aaaCGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGAc                                                                       <  2:171341/90‑1 (MQ=255)           aGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAAt                                                              <  1:763652/90‑1 (MQ=255)               aaCCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGc                                                          >  2:199629/1‑90 (MQ=255)                 ccTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGcca                                                        >  2:289185/1‑90 (MQ=255)                   tttAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGccacc                                                      <  2:318185/90‑1 (MQ=255)                         atGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑gc                                              >  2:248003/1‑90 (MQ=255)                           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑gcgc                                            <  1:113128/90‑1 (MQ=255)                           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑gcgc                                            >  2:314322/1‑90 (MQ=255)                           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑gcgc                                            >  1:65207/1‑90 (MQ=255)                           gCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑gcgc                                            >  1:72026/1‑90 (MQ=255)                              gggCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCTTc                                         >  2:904240/1‑90 (MQ=255)                                     gCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAg                                  <  1:199629/90‑1 (MQ=255)                                      ccATTTGGCGAAAATGGCAGTAACACAGGCTGGAATCCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGc                                 <  2:397902/90‑1 (MQ=255)                                        aTTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTg                               >  1:181715/1‑90 (MQ=255)                                        aTTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAA‑TCTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTg                               <  2:721202/90‑1 (MQ=255)                                                      gCAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTTGCGCGCATCGTAATAGCTGGTAAAGGGGTcc                   <  2:802666/90‑1 (MQ=255)                                                       cAGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGc                <  1:307417/90‑1 (MQ=255)                                                        aGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAACTCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGca               <  1:175443/90‑1 (MQ=255)                                                        aGTAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGca               <  2:785667/90‑1 (MQ=255)                                                          tAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGcacc             <  1:289185/90‑1 (MQ=255)                                                          tAACACAGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGcacc             <  2:65207/90‑1 (MQ=255)                                                                aGGCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAac       >  2:217216/1‑90 (MQ=255)                                                                 ggCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAaca      >  2:357836/1‑90 (MQ=255)                                                                  gCTGGAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAacac     <  2:181715/90‑1 (MQ=255)                                                                     ggAAACCTAA‑TTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACtgt  >  1:887016/1‑90 (MQ=255)                                                                               |                                                                                   AAAACGATTAAGAAAACCTTTAATATGCTGGGCTTTGCCATTTGGCGAAAATGGCAGTAACACAGGCTGGAAACCTAATTTTTCGACCAGACGGACAAAATCCGCCACCACTT‑‑GCGCATCGTAATAGCTGGTAAAGGGGTCCTGCACCACCAACACTGT  >  NZ_CP009273/1760423‑1760581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑