Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,812,807	(C)7→8	intergenic (‑50/+55)	celF ← / ← chbR	6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,812,800	1	.	C	100.0%	166.3 / NA	47	intergenic (‑43/+62)	celF/chbR	6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/24);  total (23/24)

TAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATT  >  NZ_CP009273/1812711‑1812884                                                                                           |                                                                                      taGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTg                                                                                        >  1:546088/1‑90 (MQ=255)        tCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccccccc                                                                               >  1:198604/1‑90 (MQ=255)        tCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑ccccccc                                                                               >  1:226555/1‑90 (MQ=255)         cccGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cccccccc                                                                              <  1:103095/90‑2 (MQ=255)         cccGCCACCAATAGTGACGACTCTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑cccccccc                                                                              <  1:879004/90‑2 (MQ=255)                 caATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACataa                                                                       >  1:814134/1‑90 (MQ=255)                   aTAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACataaat                                                                     >  2:852966/1‑90 (MQ=255)                    tAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACataaata                                                                    <  1:649317/90‑1 (MQ=255)                    tAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACataaata                                                                    <  1:547682/90‑1 (MQ=255)                    tAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACataaata                                                                    <  2:25185/90‑1 (MQ=255)                         acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGTTATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               <  1:760008/90‑1 (MQ=255)                         acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               <  2:757658/90‑1 (MQ=255)                         acgacTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               >  2:804032/1‑90 (MQ=255)                            acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCa                                                            <  2:272349/90‑1 (MQ=255)                            acTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCa                                                            <  1:648167/90‑1 (MQ=255)                              ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAAc                                                          <  2:132836/90‑1 (MQ=255)                              ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAAc                                                          <  1:141474/90‑1 (MQ=255)                              ttttAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAAc                                                          <  2:546088/90‑1 (MQ=255)                                  aaTTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCCGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGa                                                      <  1:861812/90‑1 (MQ=255)                                   aTTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGAc                                                     <  2:793787/90‑1 (MQ=255)                                               tAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAAt                                         <  2:630401/90‑1 (MQ=255)                                                 aTTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTg                                       <  2:198604/90‑1 (MQ=255)                                                    tctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATTTGAATTGTCa                                    <  2:731545/90‑1 (MQ=255)                                                    tctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa                                    <  2:384359/90‑1 (MQ=255)                                                    tctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCa                                    <  1:322051/90‑1 (MQ=255)                                                     ctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAg                                   <  1:169141/90‑1 (MQ=255)                                                     ctcCCTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAg                                   <  2:452335/90‑1 (MQ=255)                                                       cccTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGt                                 >  2:499710/1‑90 (MQ=255)                                                         cTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAAc                                                          <  2:894494/63‑1 (MQ=255)                                                         cTTCAGTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAAc                                                          >  1:894494/1‑63 (MQ=255)                                                              gTACAGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGa                          >  2:810216/1‑90 (MQ=255)                                                                  aGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               >  2:220956/1‑49 (MQ=255)                                                                  aGAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               <  1:220956/49‑1 (MQ=255)                                                                   gAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               <  2:292040/48‑1 (MQ=255)                                                                   gAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCa                                                               >  1:292040/1‑48 (MQ=255)                                                                   gAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                     >  2:691061/1‑90 (MQ=255)                                                                   gAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTAc                     >  2:772253/1‑90 (MQ=255)                                                                   gAATACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAACCCAGCAACAGGAAAGATTTGGGAATTTTCAGGAATAACGACttct                     >  2:625664/1‑88 (MQ=255)                                                                    aaTACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACt                    <  2:272998/90‑1 (MQ=255)                                                                    aaTACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACt                    <  2:632911/90‑1 (MQ=255)                                                                     aTACTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTg                   >  1:803487/1‑90 (MQ=255)                                                                        cTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGAtt                >  2:856489/1‑90 (MQ=255)                                                                        cTGATATCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGAtt                >  1:680686/1‑90 (MQ=255)                                                                             atCTGGCATATCTGCCCCCCCCCGGAAATAAAAAATCCAGAAAAAGGAAAGTATTGTGAATTTCCAGGAAAACCAACTTACT‑ATTaaaat           >  2:349615/1‑88 (MQ=255)                                                                             atCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  1:318701/1‑90 (MQ=255)                                                                             atCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  2:378173/1‑90 (MQ=255)                                                                             atCTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAAtt           >  2:252129/1‑90 (MQ=255)                                                                               cTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCa         >  1:221674/1‑90 (MQ=255)                                                                               cTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCa         >  1:198148/1‑90 (MQ=255)                                                                               cTGGCATATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGCATAACGACTTACTGATTAAATTCa         >  1:318447/1‑90 (MQ=255)                                                                                 ggCACATCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGt       >  1:850731/1‑90 (MQ=255)                                                                                      atCTGC‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAAtt  >  1:647037/1‑90 (MQ=255)                                                                                           |                                                                                      TAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTG‑‑CCCCCCCGGACATAAATAATCCAGCAACAGGACAGATATGTGAATTGTCAGGTATAACGACTTACTGATTAAATTCAGTCAATT  >  NZ_CP009273/1812711‑1812884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑