| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 6,994 | 0 | A | C | 61.1% | 7.7 / 16.4 | 18 | G322G (GGT→GGG) | yaaJ | sodium:alanine symporter family protein |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/11); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCAGCACCCCAGGAACCC‑ATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCG > NZ_CP009273/6910‑7079 | cacAGCCCCCCAGGACCCC‑TTTAGCCCCCCCCTCTTTTTCTGGAAAACATGAATCCCTTCCAGGGGCTTGGAGGTTGTGCCGTTCCCCGc < 2:91941/88‑1 (MQ=255) aCCCCAAGAACCCAATTACCCCCCGCATCCCCTTCTGGATAAGCTAAATCCCTTCCAGCGGAATGTAGGTTGTGCCTTTCCCCGCCAGTa < 1:224266/90‑1 (MQ=255) ccAGGAACCC‑ATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAatat > 1:496389/1‑90 (MQ=255) acccc‑ATTAGCCCCCGCATCGCTTTCTGGAAAAGCTAAATCCCTCCCGGCGGCTTGTGGGTTGTCCCTTCCCCCGCCAGTAATATCAGCa < 2:310871/88‑1 (MQ=255) cc‑ATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCAtct > 2:224266/1‑88 (MQ=255) c‑ATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGgc > 1:896538/1‑90 (MQ=255) cccccGCATCCCCTTCTGAAAAAGCTAAATCCCTCCCGGCGGTTTGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGc < 2:106646/88‑1 (MQ=255) cccGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCg > 1:590899/1‑90 (MQ=255) cccGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGCTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCg > 2:293731/1‑90 (MQ=255) ccGCATCCCCTTCTGGAAAAGCTAAATCCCTCCCGGGGGTTTGTGGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGt < 2:721303/90‑1 (MQ=255) ctcccctcctGGATAACCTAAATCCCTCCCGGCGGCTTGTGGGTTGGGCCTTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAg < 2:514801/86‑1 (MQ=255) ccccccTTCTGAAAAAGCTAAATCCCTCCCGGGGGATTGTGGTTTGTGCCGTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAg < 2:153427/86‑1 (MQ=255) cccTTTTGAAAAAGCAAAATCCCTCCCGGGGGCATGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATg < 1:19635/89‑1 (MQ=255) cTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGAc > 2:765022/1‑90 (MQ=255) ggATAACCTGATTCCCTCCCAGCGGCATGTAGTTTGTCCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAggg < 1:894986/90‑1 (MQ=255) ccAGCGGCATGTAGGTTGTGCCGTTACTCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 1:45536/90‑1 (MQ=255) tggTTGTGCCGTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACaa < 2:159067/89‑1 (MQ=255) tgTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTgcg < 2:787586/89‑1 (MQ=255) | CTCAGCACCCCAGGAACCC‑ATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCG > NZ_CP009273/6910‑7079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |