| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 108,789 | 0 | T | C | 52.9% | 7.4 / 16.6 | 17 | S100G (AGC→GGC) | zapD | cell division protein ZapD |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base C (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTT > NZ_CP009273/108709‑108870 | gCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCcgccgc > 2:755059/1‑90 (MQ=255) gTCGCTGACGCACAAGAGCAATAAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGGGGAAATTAATACGCTCCCCGCCGCTTTTa < 1:510121/90‑1 (MQ=255) gTCGCGGACGCACCAGAGAAACAAACCCATCTCCCCGAAAAATTTCCCCGATACGGGGCGCGAAAATTAATCCGCCCCCCGCCGCTTTTa < 2:341481/90‑1 (MQ=255) tGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGGGGAAATTAATACGCCCCCCGCCGCTTTTAActgc < 1:340846/90‑1 (MQ=255) ccAGAGCAATCAAACGACCTCCCCGAAAAATTTCCCCGATCCGGGGGGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAA‑TTa < 2:353750/90‑1 (MQ=255) agagAAATCAAACGATCTTCACGAAAAAATTCCCCGATCCGCGGCGGGAAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAA‑TTAAt < 1:187929/90‑1 (MQ=255) agaaCAATCAAACGATCTCCACGCAAAATTTCCCCTATACGCGGCGGGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAA‑TTAAt < 2:298701/90‑1 (MQ=255) aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCa > 2:836931/1‑90 (MQ=255) aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCa > 2:512340/1‑90 (MQ=255) cAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAAt > 2:416243/1‑90 (MQ=255) cAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAAt > 2:727445/1‑90 (MQ=255) aaCGATCTTCACGCAAAAATTGCCCGATACGCGGCGCGAAAATTAATACGCCCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATAc < 1:550250/90‑1 (MQ=255) aCGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACg > 1:560801/1‑90 (MQ=255) cGCAAAAATTCCCCGATACGCGGCGGGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACGGCTCTGGTcc < 2:140441/90‑1 (MQ=255) gCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCa > 1:140441/1‑90 (MQ=255) ggaaaaTTAATCCGCCCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCt < 1:888439/87‑1 (MQ=255) aaTACCCCCCCCGCCGCTTTTAACTGCTGAATTTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGttt < 1:80630/90‑1 (MQ=255) | GCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAA‑TTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGAGTTT > NZ_CP009273/108709‑108870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |