| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 55.6% | 7.9 / 26.0 | 18 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (2/6); new base C (10/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760098‑760276 | ggTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTCCGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGTCCCGATCCCg < 2:652469/90‑1 (MQ=255)ggTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCg < 1:615199/90‑1 (MQ=255) tGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTa < 2:544425/90‑1 (MQ=255) aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGGCCCCAACCCCGGCTCTTACtttt > 1:514076/1‑89 (MQ=255) aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCCTCCCCGGCTCTAACcttt > 1:282928/1‑89 (MQ=255) aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGAGCCGCTCCCCGGCTCTTACcttt > 2:749755/1‑89 (MQ=255) ggTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTTACTTTTTCGACAt > 1:82259/1‑90 (MQ=255) tAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGAGCCCCTCCCCGGGTCTTACTTTTTCGGCAAtct > 2:294684/1‑90 (MQ=255) gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCAACCCCGGGCCTTACTTTTTTTACATTCTCCaaaa > 2:694878/1‑89 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGt < 2:813001/90‑1 (MQ=255) aCGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAg < 2:585756/90‑1 (MQ=255) aCGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCCTCCCCGGCTCCAACTTTTTCGACATTCTCGTAATGTTCGaaaaaa > 2:295467/1‑89 (MQ=255) cTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGAt < 1:551413/90‑1 (MQ=255) gtgtCGGTATCGGCGGGGGCCCCCTCCCCGGCCCTAACTTTTTTTACATTTTCCCAATGTTTGGAAAAAAACCCCCGACCCAagcgagcg > 2:863858/1‑90 (MQ=255) gTATCGGCGGTGGCCCGATCCCCGGCTCTAACTTTTTCGACATTCTCGAAAAGTTTGAAAAAAAACCGCAGACCGAAACGATCGtgatga > 1:899726/1‑90 (MQ=255) tCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCg < 2:514076/90‑1 (MQ=255) tCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCg < 2:282928/90‑1 (MQ=255) cggcggGGACCCCCTCCCCGGGGCTAACTTTTTTGACATTTTCGAAAAGGTTGAAAAAAAACCGCAGACCGAAGCGAACGGGATGATCgg > 2:819338/1‑90 (MQ=255) cGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGACCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcg > 2:791799/1‑90 (MQ=255) gATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg > 1:354580/1‑90 (MQ=255) gggCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAgcgc < 1:335229/90‑1 (MQ=255) | GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760098‑760276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |