| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 859,169 | 0 | T | G | 56.2% | 8.4 / 12.0 | 16 | G24G (GGT→GGG) | fsa | fructose‑6‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (9/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTAACCCAAGCATTATCGCCGCGGGTAAAAAACCGCTGGATGTTGTGCTTCCGCAACTTCATGAAGCG > NZ_CP009273/859083‑859244 | aTTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGtgtg < 2:149592/90‑1 (MQ=255) ttttGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGggtgg > 1:289233/1‑89 (MQ=255) ggATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTa < 1:691269/90‑1 (MQ=255) ggTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGtgtg < 1:35997/79‑1 (MQ=255) ggTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGtgtg > 2:35997/1‑79 (MQ=255) ggTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGGGGCCACTAAacc > 2:200815/1‑90 (MQ=255) cTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGGGACCACTAACCCAAGCATTATc > 2:704910/1‑90 (MQ=255) tGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGGCCACTAAAACAAGCATTATcg > 2:742511/1‑90 (MQ=255) cTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTAACCCAAGCATTATCGCcgcg < 1:745736/90‑1 (MQ=255) gATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGGCGCgggg > 1:607053/1‑89 (MQ=255) aCTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGCCGCGGGGaaa > 1:272830/1‑90 (MQ=255) aCTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGCCGCGGGGaaa > 2:880650/1‑90 (MQ=255) tCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGCCGCGGGGaaaaaa > 1:301335/1‑90 (MQ=255) cAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCCCCAACCCAAGCATTATCGCCGCGGGGAAAAAAc > 2:634278/1‑90 (MQ=255) gACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTa < 1:873880/58‑1 (MQ=255) gACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTa > 2:873880/1‑58 (MQ=255) gACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGCCGCGGGGAAAAAACCg > 1:49827/1‑90 (MQ=255) tgttgCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTAACCCAAGCATTATCGCCGCGGGGAAAAAACCGCTGGa > 2:587991/1‑90 (MQ=255) tgttgCGGTGAAGGCGCTGCCACGTATTTTTCCGCTGGCGGGGGTGACCACTAACCCAAGCATTATCGCCGCGGGGAAAAAACCGCTggg > 2:377274/1‑89 (MQ=255) tttCCGCTGGCGGGGGGGACCACTAACCCAAGCATTATCGCCGCGGGTAAAAAACCGCTGGCTGTTTTGCTTCCGCCACTTCTTGAAGCg > 1:838472/1‑90 (MQ=255) | ATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGCGCTGTCACGTATTTTTCCGCTGGCGGGTGTGACCACTAACCCAAGCATTATCGCCGCGGGTAAAAAACCGCTGGATGTTGTGCTTCCGCAACTTCATGAAGCG > NZ_CP009273/859083‑859244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |