| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,872,730 | 0 | C | G | 54.2% | 2.5 / 37.3 | 24 | G253G (GGC→GGG) | dgcP | diguanylate cyclase DgcP |
| Reads supporting (aligned to +/- strand): ref base C (7/4); new base G (8/5); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGG > NZ_CP009273/1872648‑1872816 | ataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGtgatg < 2:424502/90‑1 (MQ=255)ataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGtgatg < 2:516821/90‑1 (MQ=255) gTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGt > 1:819765/1‑90 (MQ=255) gTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGt > 1:479055/1‑90 (MQ=255) ggggATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGttt > 1:159440/4‑90 (MQ=255) gATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTa > 1:310614/1‑90 (MQ=255) gATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTa > 1:449467/1‑90 (MQ=255) gATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGGGATGAGGTTTTa > 1:266223/1‑90 (MQ=255) gTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTAgttgt < 2:819765/90‑1 (MQ=255) ttCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTAgttgt < 1:400089/82‑1 (MQ=255) ttCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTAgttgt > 2:400089/1‑82 (MQ=255) ttCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTaa > 1:447035/1‑90 (MQ=255) tCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTAGTTGTTTCACTaaa < 1:104471/90‑1 (MQ=255) ggTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGGGATGAGGTTTTTGTTGTTTCACCAaacaa > 1:687506/1‑90 (MQ=255) gcgcCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGGGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGc < 1:753828/90‑1 (MQ=255) cTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTtcgtcg > 1:202872/1‑90 (MQ=255) cTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGGGGTGAGCTTTTAGTTGTTTTGCCTAATAAACAGAATTCCGAGATTtcgtcg > 1:370647/1‑90 (MQ=255) aGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTTGTTGTTTTACTAAAAAACGAGAATGCGGGTATTTCGTCGCGGc > 2:76441/1‑90 (MQ=255) ggCGAAGTTATTGGTCGTCTCGGCGGTGGTGAGTTTTTAGTTGTTTCAGTAAACAACGAGAAAGCGGATATTTCGTCGCTGCGAGGACGc > 2:44727/1‑90 (MQ=255) cGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCAt < 1:93073/90‑1 (MQ=255) ttATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTcagc < 2:202872/90‑1 (MQ=255) gtcgtcTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCaaa < 2:605926/90‑1 (MQ=255) cgtcTCGGGGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATa > 1:3917/1‑90 (MQ=255) gtcTCGGGGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATAc > 2:229646/1‑90 (MQ=255) cggcggTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTgg < 2:447035/90‑1 (MQ=255) | ATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGG > NZ_CP009273/1872648‑1872816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |