| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,002,571 | 0 | A | G | 53.3% | 5.7 / 12.9 | 15 | I272V (ATC→GTC) | yedE | selenium metabolism membrane protein YedE/FdhT |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base G (8/0); total (12/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGG > NZ_CP009273/2002484‑2002659 | aCTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGAGTGTCATCTGGAta > 1:782908/1‑89 (MQ=255) cTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCt < 2:759396/90‑1 (MQ=255) aGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGGGGGGCCTCGGGGTCTTCAg > 1:29934/1‑90 (MQ=255) cACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGAGTGCCCTCGGGGTCTTTCGTTACGTACAGTTAGGGGTTGa > 1:262646/1‑90 (MQ=255) ccGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGGGGGCCATCGGGGTTTTTAGTTACGTACAATTAGGGGTTGaaa > 2:1317/1‑89 (MQ=255) tACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGGGGGCCCCCGGGGTCTTTAGGTAACTACAGGTAGGCGGTGAACCCAAAAt > 2:818281/1‑90 (MQ=255) ccATATGGCGAAAGCAATCATTATCGGTATGGCGGGGAGGGCCCTCGGGGGGTTCCGTTTAGTACAGTTTGGGGTTGAACCCCAAAACAt > 2:688237/1‑90 (MQ=255) tatGGCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCATCGGGGTCTTCAGTTACGTACAGTTAGGGGTTGAAACCAAAATCAtgtg > 1:574960/1‑90 (MQ=255) gAAAGCAATCATTATCGGTATGGCGGTGAGGGCCCTCGGGGTCTTTAGTTACGGAAAGTTAGGGGGTGAACCCAAAAACATGTGgggggg > 2:313531/1‑90 (MQ=255) aTCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTa > 1:753535/1‑54 (MQ=255) aTCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTa < 2:753535/54‑1 (MQ=255) aTCATTATCGGTATGGCGGGGGGGGCCCTCGGGGTCTTCAACTACATACAGATATGCGTTTAAACCAAAAATATGTGGGCGGGGCCAAAc > 2:353821/1‑90 (MQ=255) gTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCCAAATCATGTGGGGGGGACCCAACGCGGTAAttt > 1:453045/1‑89 (MQ=255) tATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATtgg < 1:160149/90‑1 (MQ=255) gATCATCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTgg > 2:725108/1‑90 (MQ=255) | ACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGG > NZ_CP009273/2002484‑2002659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |